| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 61 | Sinorhizobium fredii | AGCCTCCGCATCAACGAA | ATTTGGCGTGGCTGTCGA | 59.34 | 59.97 | 235 | 64 |
100.00%
|
100.00%
|
| Primer 62 | Sinorhizobium fredii | TGGTCGTCGCCATGCAAA | CGGCATCCTCGTTGTGACT | 60.28 | 60.08 | 210 | 64 |
100.00%
|
100.00%
|
| Primer 63 | Sinorhizobium fredii | TGGTCGTCGCCATGCAAA | TCGGCATCCTCGTTGTGAC | 60.28 | 60.08 | 211 | 64 |
100.00%
|
100.00%
|
| Primer 64 | Sinorhizobium fredii | GAGAACAGTGCGAACGGGA | ATTGACGAGGCTGCCGAA | 60.01 | 59.34 | 189 | 64 |
100.00%
|
100.00%
|
| Primer 65 | Sinorhizobium fredii | AGAACAGTGCGAACGGGA | ATTGACGAGGCTGCCGAA | 58.87 | 59.34 | 188 | 64 |
100.00%
|
100.00%
|
| Primer 66 | Sinorhizobium fredii | ACAGTGCGAACGGGATCTC | ATTGACGAGGCTGCCGAA | 59.78 | 59.34 | 185 | 64 |
100.00%
|
100.00%
|
| Primer 67 | Sinorhizobium fredii | TCGGTTCGCGTGTTCGAA | ATTTCGTGCGACTCGTGC | 59.97 | 58.84 | 241 | 64 |
100.00%
|
100.00%
|
| Primer 68 | Sinorhizobium fredii | TCGGTTCGCGTGTTCGAA | TTCGTGCGACTCGTGCA | 59.97 | 59.61 | 239 | 64 |
100.00%
|
100.00%
|
| Primer 69 | Sinorhizobium fredii | TGCGGATGAAATCGCACG | GCGAAATGCGACGACGAA | 58.91 | 59.23 | 208 | 64 |
100.00%
|
100.00%
|
| Primer 70 | Sinorhizobium fredii | TTCGGCAGCCTCGTCAAT | ATGAGGAAGAAGGCGAGCG | 59.34 | 59.86 | 257 | 60 |
100.00%
|
100.00%
|