| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Thermobifida fusca | TGGTGGCGATGATCCTTCC | TTCTTCGTCGTCTGGGCTG | 59.47 | 59.71 | 167 | 160 |
100.00%
|
100.00%
|
| Primer 2 | Thermobifida fusca | TCACGTTCTCCGCTGAAGG | AGGGAACGCACGATGAAGG | 59.71 | 60.08 | 175 | 144 |
100.00%
|
100.00%
|
| Primer 3 | Thermobifida fusca | AGCTCGCCAACGACAAGT | TTCGCGTGGGTGATGATGG | 59.58 | 60.45 | 181 | 143 |
100.00%
|
100.00%
|
| Primer 4 | Thermobifida fusca | AGCTCGCCAACGACAAGT | ATGGAGCCACCGAAGATGG | 59.58 | 59.47 | 166 | 143 |
100.00%
|
100.00%
|
| Primer 5 | Thermobifida fusca | AGCTCGCCAACGACAAGT | CCTTCGCGTGGGTGATGAT | 59.58 | 60.15 | 183 | 141 |
100.00%
|
100.00%
|
| Primer 6 | Thermobifida fusca | TGAACACGAGCAGCGCAT | AAGACCATGCCTCCCAGGA | 60.36 | 60.23 | 174 | 140 |
100.00%
|
100.00%
|
| Primer 7 | Thermobifida fusca | ACCGCAAAATCGTGCAGC | TTCGAAGGTCAGCGAGGTG | 59.74 | 59.71 | 194 | 140 |
100.00%
|
100.00%
|
| Primer 8 | Thermobifida fusca | TACGCCAGCGGAGTTTGT | ACGCTTCGACGAGGGAAAG | 59.26 | 60.08 | 164 | 140 |
100.00%
|
100.00%
|
| Primer 9 | Thermobifida fusca | TGCCGATACCGACAAGAGC | AACCGCTTCTGCTGCCAT | 59.86 | 59.97 | 226 | 138 |
100.00%
|
100.00%
|
| Primer 10 | Thermobifida fusca | ACGGTACCAGCTTTCGCA | AGCCACAATGAGCCGTACC | 59.26 | 60.08 | 265 | 138 |
100.00%
|
100.00%
|