| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1141 | Thermobifida fusca | TCGACGTCAAAGCCGACTC | TCTGCACGCAGGAAACGT | 60.08 | 59.89 | 237 | 79 |
100.00%
|
100.00%
|
| Primer 1142 | Thermobifida fusca | AACCGAGTTCCTGCCGATC | TGCTGCCGATGATGACGT | 59.78 | 59.42 | 242 | 79 |
100.00%
|
100.00%
|
| Primer 1143 | Thermobifida fusca | TGCGCTGAGTGTGACTGT | ACGAAGATCACCACGACCG | 59.19 | 59.79 | 240 | 79 |
100.00%
|
100.00%
|
| Primer 1144 | Thermobifida fusca | CTGCGCTGAGTGTGACTGT | ACGAAGATCACCACGACCG | 60.30 | 59.79 | 241 | 79 |
100.00%
|
100.00%
|
| Primer 1145 | Thermobifida fusca | TGCGCTGAGTGTGACTGTG | GCCACCACGAAGATCACCA | 60.59 | 60.00 | 246 | 79 |
100.00%
|
100.00%
|
| Primer 1146 | Thermobifida fusca | TCACCGTCGCAAAAGCCA | TGCTCGGCTTCGAACCAT | 60.20 | 59.34 | 283 | 79 |
100.00%
|
100.00%
|
| Primer 1147 | Thermobifida fusca | GTGCACGCGTTGACTGAA | CTGGTGAACAGCCACTCGT | 58.99 | 59.93 | 215 | 79 |
100.00%
|
100.00%
|
| Primer 1148 | Thermobifida fusca | TAGGCATCCCCTGGTTCGT | CCACCAGAAGCAGCATCCA | 60.31 | 60.00 | 270 | 79 |
100.00%
|
100.00%
|
| Primer 1149 | Thermobifida fusca | GCTTGACCATCAGCGACCT | TGAACAGGGCTTTGGCGT | 60.08 | 59.81 | 183 | 79 |
100.00%
|
100.00%
|
| Primer 1150 | Thermobifida fusca | AGCTTGACCATCAGCGACC | TGAACAGGGCTTTGGCGT | 60.08 | 59.81 | 184 | 79 |
100.00%
|
100.00%
|