| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1331 | Thermobifida fusca | TACCTCCTTCAGCAGCGGA | TGAGCGCTTCAAACAGGC | 60.30 | 58.66 | 265 | 73 |
100.00%
|
100.00%
|
| Primer 1332 | Thermobifida fusca | TCGACGACCACGACTATGC | TAGGTGTCGTCGTAGCGGA | 59.57 | 60.08 | 268 | 73 |
100.00%
|
100.00%
|
| Primer 1333 | Thermobifida fusca | TCAACACGTCGCACTGGA | CGAGCGAACAAAAGTGCCC | 59.19 | 60.08 | 216 | 73 |
100.00%
|
100.00%
|
| Primer 1334 | Thermobifida fusca | TCCGTGAAACCGTCCAAGG | GCGCATCGTAGAACCAGGA | 59.93 | 59.86 | 186 | 73 |
100.00%
|
100.00%
|
| Primer 1335 | Thermobifida fusca | ACGAGATCACCCTCCGTGT | TTCGCCTTGCCCTTTGGA | 60.30 | 59.48 | 215 | 73 |
100.00%
|
100.00%
|
| Primer 1336 | Thermobifida fusca | GAACACCACCAACACGCAC | ACGCGTGGAAATGGTGGA | 59.94 | 59.57 | 185 | 73 |
100.00%
|
100.00%
|
| Primer 1337 | Thermobifida fusca | AACACCACCAACACGCAC | ACGCGTGGAAATGGTGGA | 58.81 | 59.57 | 184 | 73 |
100.00%
|
100.00%
|
| Primer 1338 | Thermobifida fusca | GCAAAATCGTGCAGCGGA | TTCGAAGGTCAGCGAGGTG | 59.44 | 59.71 | 191 | 73 |
100.00%
|
100.00%
|
| Primer 1339 | Thermobifida fusca | ACCATCGAACGCTTCTCCG | TAGACGAGGATGCAGGGCT | 60.15 | 60.08 | 194 | 72 |
100.00%
|
100.00%
|
| Primer 1340 | Thermobifida fusca | TGGTGGCGATGATCCTTCC | TTCTTCGTCGTCTGGGCT | 59.47 | 58.23 | 167 | 72 |
100.00%
|
100.00%
|