| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1491 | Thermobifida fusca | TGTCCGGTGTCAGCATTCC | TGTCCTTGGGAATCTCCGC | 60.00 | 59.40 | 165 | 69 |
100.00%
|
100.00%
|
| Primer 1492 | Thermobifida fusca | GCGTGGTACAAGATTGCGC | GGTGCAATCCGTCCTCCAA | 60.23 | 60.00 | 169 | 69 |
100.00%
|
100.00%
|
| Primer 1493 | Thermobifida fusca | TCGGCTCGCTGAAACTGA | ACGCATCCATGGCAGCTT | 58.95 | 60.04 | 203 | 69 |
100.00%
|
100.00%
|
| Primer 1494 | Thermobifida fusca | TTCGGGAATCTCGACACGC | TGAGCACTTGTGCAGCCA | 60.15 | 59.81 | 283 | 69 |
100.00%
|
100.00%
|
| Primer 1495 | Thermobifida fusca | AGTCGAGATCACCGCAACC | ATCGCTGGATCTCCGCTT | 59.78 | 58.46 | 272 | 69 |
100.00%
|
100.00%
|
| Primer 1496 | Thermobifida fusca | ACCTCAAAGCCTTCCTCGC | TGCCGAACTTCGCGTCTT | 60.00 | 59.97 | 183 | 69 |
100.00%
|
100.00%
|
| Primer 1497 | Thermobifida fusca | TTGGCTGAGACCCTGGAGA | ATTCCCTGGGAAGAGGCCA | 59.84 | 60.23 | 173 | 69 |
100.00%
|
100.00%
|
| Primer 1498 | Thermobifida fusca | AGCACGAACCGACGAGATC | TGTTGCCGTGCACGTAGA | 59.86 | 59.58 | 218 | 69 |
100.00%
|
100.00%
|
| Primer 1499 | Thermobifida fusca | GAGCACGAACCGACGAGAT | TGTTGCCGTGCACGTAGA | 59.86 | 59.58 | 219 | 69 |
100.00%
|
100.00%
|
| Primer 1500 | Thermobifida fusca | AGCACGAACCGACGAGAT | TGTTGCCGTGCACGTAGA | 58.72 | 59.58 | 218 | 69 |
100.00%
|
100.00%
|