| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 2471 | Thermobifida fusca | TGGATGGAACGCTGGCCTA | TTCGTGTCCAACACCGCA | 60.99 | 59.81 | 257 | 44 |
100.00%
|
100.00%
|
| Primer 2472 | Thermobifida fusca | TCACGCCAAGCAACCAGA | AGGTTGAGCACGTTGCCT | 59.49 | 59.49 | 187 | 44 |
100.00%
|
100.00%
|
| Primer 2473 | Thermobifida fusca | ACTCGAAGCACTGCGGTT | ATTCCCAGGTGTGCAGCA | 59.58 | 59.15 | 296 | 44 |
100.00%
|
100.00%
|
| Primer 2474 | Thermobifida fusca | AACGGGACTTTGCGGTGA | TCGACAAACCTGCTGGCA | 59.49 | 59.49 | 175 | 43 |
100.00%
|
100.00%
|
| Primer 2475 | Thermobifida fusca | TGTTCGCGTTCTTCCCTCC | GGCGAGGAAGACCAGTTCA | 60.01 | 59.33 | 215 | 43 |
100.00%
|
100.00%
|
| Primer 2476 | Thermobifida fusca | CCGACCATGCTGATCGTGA | TTGTGGCCATGAGCAGCA | 59.86 | 59.88 | 151 | 43 |
100.00%
|
100.00%
|
| Primer 2477 | Thermobifida fusca | TCCTGGAAGCCCAAGGAGT | GAGTTCGTCACCACGGTCA | 60.15 | 59.64 | 232 | 43 |
100.00%
|
100.00%
|
| Primer 2478 | Thermobifida fusca | ATGCGCACCTACCGTTCA | AGCCGAAGCGTTGCATGA | 59.34 | 60.36 | 259 | 43 |
100.00%
|
100.00%
|
| Primer 2479 | Thermobifida fusca | CACACGGTTTCTGGGCTGA | AGCCCAACTGTCGTCGAAG | 60.23 | 60.01 | 210 | 43 |
100.00%
|
100.00%
|
| Primer 2480 | Thermobifida fusca | CTCGTGTTCGGCTTCCTGT | ACGAGGTGGTCAATGGACG | 60.01 | 59.71 | 215 | 43 |
100.00%
|
100.00%
|