| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 591 | Thermobifida fusca | AGTCGAGATCACCGCAACC | GCTGGATCTCCGCTTCGAT | 59.78 | 59.63 | 269 | 97 |
100.00%
|
100.00%
|
| Primer 592 | Thermobifida fusca | ACTTTGCACCCCAGCACA | TCCCACAGCATGAAGACGG | 59.72 | 59.70 | 283 | 97 |
100.00%
|
100.00%
|
| Primer 593 | Thermobifida fusca | TGACCGTCAACACCGCAA | TGAACGCGTTGGCTTTGC | 59.81 | 59.97 | 153 | 97 |
100.00%
|
100.00%
|
| Primer 594 | Thermobifida fusca | CCATGCCGTTCCTGGTGAT | TGCGCTGCATCTCCAAGT | 60.08 | 59.65 | 267 | 97 |
100.00%
|
100.00%
|
| Primer 595 | Thermobifida fusca | ACCGAACGTCCCGATCTTG | TCGATCCGGTTGGTCATGG | 59.79 | 59.48 | 233 | 97 |
100.00%
|
100.00%
|
| Primer 596 | Thermobifida fusca | ACCGAACGTCCCGATCTTG | ATCCGGTTGGTCATGGTCC | 59.79 | 59.39 | 230 | 97 |
100.00%
|
100.00%
|
| Primer 597 | Thermobifida fusca | TGGCTGATTTTCGCGTGC | CGAACGAGTCCTGCGTGAT | 59.44 | 60.15 | 208 | 97 |
100.00%
|
100.00%
|
| Primer 598 | Thermobifida fusca | TGGCTGATTTTCGCGTGC | ACTCCACATTGCCGAGGAC | 59.44 | 59.70 | 187 | 97 |
100.00%
|
100.00%
|
| Primer 599 | Thermobifida fusca | GCTTTCCTGCAGCAGTTGAC | AACACTGCCGATCGCGAT | 60.04 | 59.82 | 249 | 97 |
100.00%
|
100.00%
|
| Primer 600 | Thermobifida fusca | TCGTTCGTCTCCGCTTCAG | ACGCAGAGCTTCCGAAGAC | 59.79 | 60.08 | 196 | 97 |
100.00%
|
100.00%
|