| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 961 | Thermobifida fusca | TGCTGACGGAATGGGTGTC | TCTTGAACGCCAGCAGGA | 60.00 | 58.85 | 210 | 84 |
100.00%
|
100.00%
|
| Primer 962 | Thermobifida fusca | TGGAGAGGTTGGACACGGA | TGGTCCCCTTCGAATTCTGC | 60.15 | 60.04 | 202 | 84 |
100.00%
|
100.00%
|
| Primer 963 | Thermobifida fusca | TGCCGACCGTTGAATACCC | ACAGAATGCAGCGCAGGT | 60.08 | 59.97 | 240 | 84 |
100.00%
|
100.00%
|
| Primer 964 | Thermobifida fusca | ACCGCAAAATCGTGCAGC | CTTCGAAGGTCAGCGAGGT | 59.74 | 59.41 | 195 | 84 |
100.00%
|
100.00%
|
| Primer 965 | Thermobifida fusca | TGCTCACATCCATCGTCCG | TCGCCTCGTACTTAGGGGT | 59.86 | 59.70 | 234 | 84 |
100.00%
|
100.00%
|
| Primer 966 | Thermobifida fusca | AAGCTCGCGAACGCATCT | TCATCATCGGCAGCGCAT | 60.13 | 59.89 | 198 | 83 |
100.00%
|
100.00%
|
| Primer 967 | Thermobifida fusca | ACGAGCTTGCCGACTTCT | CCAGCGGAACCCATTCGAT | 58.95 | 60.15 | 153 | 83 |
100.00%
|
100.00%
|
| Primer 968 | Thermobifida fusca | AGCGCATCGGCAGTTTCT | GCTAGCTCAGCCATGCTCA | 60.05 | 59.85 | 277 | 83 |
100.00%
|
100.00%
|
| Primer 969 | Thermobifida fusca | AGCGCATCGGCAGTTTCT | ATCTCGTGCATGAGGTGGC | 60.05 | 60.15 | 202 | 83 |
100.00%
|
100.00%
|
| Primer 970 | Thermobifida fusca | AGCGCATCGGCAGTTTCT | TCAGCCATGCTCATGTCGG | 60.05 | 60.15 | 271 | 83 |
100.00%
|
100.00%
|