| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1311 | Thermobispora bispora | AGCATCGACAGCAGGTACG | GGACGATGCCGACGAAGAT | 59.86 | 59.93 | 244 | 63 |
100.00%
|
100.00%
|
| Primer 1312 | Thermobispora bispora | GGCGAGGGCATGAAGATCA | TTGATCTGCTCGACGACCG | 59.85 | 59.86 | 242 | 63 |
100.00%
|
100.00%
|
| Primer 1313 | Thermobispora bispora | ACGTGCGCATGTTCCTGA | GACTGGACGAACCGCTTGA | 59.97 | 60.01 | 160 | 63 |
100.00%
|
100.00%
|
| Primer 1314 | Thermobispora bispora | TGGCACCTGCACTTCCA | TTACGGGAGGTGTCCACGA | 58.64 | 60.23 | 161 | 63 |
100.00%
|
100.00%
|
| Primer 1315 | Thermobispora bispora | TGGCACCTGCACTTCCA | GTTACGGGAGGTGTCCACGA | 58.64 | 61.53 | 162 | 63 |
100.00%
|
100.00%
|
| Primer 1316 | Thermobispora bispora | CGGGTGCTGTGGAAGATGT | GGAACTTGGCGAGCTCCAT | 60.00 | 60.08 | 244 | 63 |
100.00%
|
100.00%
|
| Primer 1317 | Thermobispora bispora | TGGTGGTCAACGACGTGA | ACATCTTCCACAGCACCCG | 58.79 | 60.00 | 210 | 63 |
100.00%
|
100.00%
|
| Primer 1318 | Thermobispora bispora | TCGTGATCGCCACCCATGA | TCGCTGAAGGCCACGTA | 61.36 | 58.18 | 238 | 63 |
100.00%
|
100.00%
|
| Primer 1319 | Thermobispora bispora | TGATCGCCAATGCCGTG | TGGTGCGCATGTAGCTCTT | 58.06 | 59.70 | 222 | 63 |
100.00%
|
100.00%
|
| Primer 1320 | Thermobispora bispora | ATGCCGAGCAGATGCCTT | TGCGACCGTCTGTGTGAT | 59.41 | 58.95 | 283 | 63 |
100.00%
|
100.00%
|