| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 41 | Thermosipho africanus | AGGCAAATCCATAGGAGTCCA | TTTTGAAGCGCATCCGGAT | 58.51 | 58.13 | 171 | 135 |
100.00%
|
100.00%
|
| Primer 42 | Thermosipho africanus | AGGCAAATCCATAGGAGTCCA | ATTTTGAAGCGCATCCGGA | 58.51 | 58.13 | 172 | 135 |
100.00%
|
100.00%
|
| Primer 43 | Thermosipho africanus | ACGACTGGGGAAGGCAAAG | GGTACAGCTACTGCAAATCCA | 59.93 | 58.01 | 160 | 135 |
100.00%
|
100.00%
|
| Primer 44 | Thermosipho africanus | ACGACTGGGGAAGGCAAA | GGTACAGCTACTGCAAATCCA | 58.75 | 58.01 | 160 | 135 |
100.00%
|
100.00%
|
| Primer 45 | Thermosipho africanus | TACGACTGGGGAAGGCAAAG | GGTACAGCTACTGCAAATCCA | 59.68 | 58.01 | 161 | 135 |
100.00%
|
100.00%
|
| Primer 46 | Thermosipho africanus | TACGACTGGGGAAGGCAAA | GGTACAGCTACTGCAAATCCA | 58.55 | 58.01 | 161 | 135 |
100.00%
|
100.00%
|
| Primer 47 | Thermosipho africanus | TTTACGACTGGGGAAGGCA | GGTACAGCTACTGCAAATCCA | 58.55 | 58.01 | 163 | 135 |
100.00%
|
100.00%
|
| Primer 48 | Thermosipho africanus | CGACTGGGGAAGGCAAAGAG | GGTACAGCTACTGCAAATCCA | 60.67 | 58.01 | 159 | 135 |
100.00%
|
100.00%
|
| Primer 49 | Thermosipho africanus | TTACGACTGGGGAAGGCAAA | GGTACAGCTACTGCAAATCCA | 59.23 | 58.01 | 162 | 135 |
100.00%
|
100.00%
|
| Primer 50 | Thermosipho africanus | AAGTGGGTTGAGCAAGGGC | GCCATCATTCAACGCCTTCC | 60.53 | 59.90 | 243 | 132 |
100.00%
|
100.00%
|