| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 31 | Thermotoga neapolitana | ACTCTGTGGAGACCGCTCT | TTCCACACCTGCTTGGCAT | 59.92 | 59.85 | 181 | 127 |
100.00%
|
100.00%
|
| Primer 32 | Thermotoga neapolitana | ACTCTGTGGAGACCGCTCT | CCCATGTTCGGCAGTTCCA | 59.92 | 60.30 | 244 | 127 |
100.00%
|
100.00%
|
| Primer 33 | Thermotoga neapolitana | ACTCTGTGGAGACCGCTCT | TCCACACCTGCTTGGCATC | 59.92 | 60.30 | 180 | 127 |
100.00%
|
100.00%
|
| Primer 34 | Thermotoga neapolitana | ACTCTGTGGAGACCGCTCT | CCACACCTGCTTGGCATCT | 59.92 | 60.30 | 179 | 127 |
100.00%
|
100.00%
|
| Primer 35 | Thermotoga neapolitana | ACTCTGTGGAGACCGCTCT | TGCTTGGCATCTTCTCCCC | 59.92 | 59.70 | 172 | 127 |
100.00%
|
100.00%
|
| Primer 36 | Thermotoga neapolitana | GTTCGTTGTTCCGAGGGGA | TCGTTCGGCACACACACT | 59.63 | 59.50 | 231 | 127 |
100.00%
|
100.00%
|
| Primer 37 | Thermotoga neapolitana | TGGGGTACGTCTGGCTCAT | ACACCGAGTGCCAGCAAA | 60.31 | 59.81 | 166 | 126 |
100.00%
|
100.00%
|
| Primer 38 | Thermotoga neapolitana | TGGCGACCATCCTGAAACC | TCCCCTTCGAAGCGTTCTG | 60.00 | 59.71 | 252 | 126 |
100.00%
|
100.00%
|
| Primer 39 | Thermotoga neapolitana | CTGGAAGCTGCCGATCTGT | TGCATAAGCGCTGGCCAT | 59.78 | 60.12 | 231 | 126 |
100.00%
|
100.00%
|
| Primer 40 | Thermotoga neapolitana | TCCTTTTGCCTGCGGTGT | TCCTCTTCCGTGACGTTGC | 59.81 | 60.01 | 177 | 126 |
100.00%
|
100.00%
|