| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 31 | Thermotoga petrophila | TTCACGTTTGCTGTGCGC | TCTTGCATCGTTCGCTGC | 59.98 | 58.83 | 249 | 114 |
100.00%
|
100.00%
|
| Primer 32 | Thermotoga petrophila | TTCACGTTTGCTGTGCGC | TCTCCTCGTCGCTTCTTGC | 59.98 | 59.79 | 299 | 114 |
100.00%
|
100.00%
|
| Primer 33 | Thermotoga petrophila | TTCACGTTTGCTGTGCGC | TTCTTGCATCGTTCGCTGC | 59.98 | 59.50 | 250 | 114 |
100.00%
|
100.00%
|
| Primer 34 | Thermotoga petrophila | TTCACGTTTGCTGTGCGC | TTTCTTGCATCGTTCGCTGC | 59.98 | 60.11 | 251 | 114 |
100.00%
|
100.00%
|
| Primer 35 | Thermotoga petrophila | TGGTGTATGGGTGCTCACTC | TCCTCCACGGGATGAGAGA | 59.39 | 58.99 | 183 | 114 |
100.00%
|
100.00%
|
| Primer 36 | Thermotoga petrophila | TCGGGAAAACCCTTGCCA | GCGCATATGCTCCCAGGAT | 59.07 | 60.00 | 273 | 113 |
100.00%
|
50.00%
|
| Primer 37 | Thermotoga petrophila | TCGGGAAAACCCTTGCCA | GCTTTCCCAGCGCATATGC | 59.07 | 60.01 | 282 | 113 |
100.00%
|
50.00%
|
| Primer 38 | Thermotoga petrophila | TTCTGCGAGCGTTCACGT | TCCTTCCGCTCTGTAACCG | 59.97 | 59.11 | 256 | 112 |
100.00%
|
50.00%
|
| Primer 39 | Thermotoga petrophila | TTCTGCGAGCGTTCACGT | CCTTCCGCTCTGTAACCGA | 59.97 | 59.11 | 255 | 109 |
100.00%
|
50.00%
|
| Primer 40 | Thermotoga petrophila | TGTTTTGCCCAGGTGCCA | TCCTCAAGCCAGCAAACGA | 60.04 | 59.55 | 251 | 109 |
100.00%
|
100.00%
|