| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 21 | Thiobacillus denitrificans | ATGCGCTCACCCTTGCAA | TTTCCACCAGACCTCGCAG | 60.28 | 59.63 | 275 | 130 |
100.00%
|
100.00%
|
| Primer 22 | Thiobacillus denitrificans | TGCACTTTCGCGTCGTCA | ACTTCGACCAGACGTTCGG | 60.28 | 59.71 | 244 | 130 |
100.00%
|
100.00%
|
| Primer 23 | Thiobacillus denitrificans | TTCAGCGCAAGGATGGCA | TTCGAACAGCGCATCGGT | 59.97 | 60.05 | 209 | 128 |
100.00%
|
100.00%
|
| Primer 24 | Thiobacillus denitrificans | TGCACTTTCGCGTCGTCA | CTTCGACCAGACGTTCGGT | 60.28 | 59.71 | 243 | 128 |
100.00%
|
100.00%
|
| Primer 25 | Thiobacillus denitrificans | GCAAGGAAATGCTGCGCT | TTGGCAAACGCGTTCTGC | 59.43 | 59.97 | 193 | 128 |
100.00%
|
100.00%
|
| Primer 26 | Thiobacillus denitrificans | ACGCATTCCGAGCTGCAT | TGGCGATGATGACGAGGTC | 60.12 | 59.56 | 220 | 127 |
100.00%
|
100.00%
|
| Primer 27 | Thiobacillus denitrificans | TTCGACCGCTTCATCGCA | ACAGGATCAGGCTCGGGAT | 59.74 | 60.07 | 208 | 127 |
100.00%
|
100.00%
|
| Primer 28 | Thiobacillus denitrificans | TTCGACCGCTTCATCGCA | TCGGGATGCCCTTGATGTG | 59.74 | 59.78 | 196 | 127 |
100.00%
|
100.00%
|
| Primer 29 | Thiobacillus denitrificans | TTCGACCGCTTCATCGCA | AACAGGATCAGGCTCGGGA | 59.74 | 60.31 | 209 | 127 |
100.00%
|
100.00%
|
| Primer 30 | Thiobacillus denitrificans | TTCGACCGCTTCATCGCA | TTGCGGAACAGGATCAGGC | 59.74 | 60.38 | 215 | 127 |
100.00%
|
100.00%
|