| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 331 | Thiobacillus denitrificans | TCGCGTGACAGCCTGAAA | TCTTCACGAGCTTGCCGT | 59.58 | 59.27 | 193 | 45 |
100.00%
|
100.00%
|
| Primer 332 | Thiobacillus denitrificans | ATCGCACGAGGGCTTCAA | TCTTCACGAGCTTGCCGT | 59.34 | 59.27 | 176 | 45 |
100.00%
|
100.00%
|
| Primer 333 | Thiobacillus denitrificans | AGGCGAACATGGAAGCGT | TGGTGATCGAGCCGAACAG | 59.65 | 59.78 | 162 | 45 |
100.00%
|
100.00%
|
| Primer 334 | Thiobacillus denitrificans | AGGCGAACATGGAAGCGT | AGCACGACCGGATATTCGC | 59.65 | 60.30 | 271 | 45 |
100.00%
|
100.00%
|
| Primer 335 | Thiobacillus denitrificans | AGGCGAACATGGAAGCGT | TGGTGATCGAGCCGAACA | 59.65 | 58.63 | 162 | 45 |
100.00%
|
100.00%
|
| Primer 336 | Thiobacillus denitrificans | AGGCGAACATGGAAGCGT | CGTTGGTGATCGAGCCGAA | 59.65 | 60.45 | 165 | 45 |
100.00%
|
100.00%
|
| Primer 337 | Thiobacillus denitrificans | ACAACGGCATCGTCGTGA | TGATGGTCGACGGCAGTT | 59.66 | 58.94 | 228 | 44 |
100.00%
|
100.00%
|
| Primer 338 | Thiobacillus denitrificans | AGACGCCAAGCTGCAGTT | TGTCCATCTCGAACGGCAC | 59.89 | 60.08 | 170 | 44 |
100.00%
|
100.00%
|
| Primer 339 | Thiobacillus denitrificans | CGCAGGTGAACGAGGCTTA | TGCGCAACTCATCGGTCA | 60.08 | 59.66 | 213 | 44 |
100.00%
|
100.00%
|
| Primer 340 | Thiobacillus denitrificans | ATGAGCCTCGATCGCGTT | TCTTCAGCATGCCGATCGG | 59.19 | 60.23 | 286 | 44 |
100.00%
|
100.00%
|