| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1001 | Vibrio orientalis | TTGACCCTGACACACCCGA | TGCGCTGCTTGAACTCCA | 60.76 | 59.89 | 289 | 53 |
100.00%
|
100.00%
|
| Primer 1002 | Vibrio orientalis | CACATCGAACGTCCAGCCT | GCTTCGATCGCCTTTTCGAC | 60.08 | 59.97 | 153 | 53 |
100.00%
|
100.00%
|
| Primer 1003 | Vibrio orientalis | TGCGTGGGAGTTTGCACA | GCTCAGCCCATACCGTGTT | 60.12 | 60.08 | 264 | 53 |
100.00%
|
100.00%
|
| Primer 1004 | Vibrio orientalis | AAAGCTGCTCTGCGTGGT | TAGGTGCGCTTGGAACTGG | 59.89 | 60.00 | 201 | 53 |
100.00%
|
100.00%
|
| Primer 1005 | Vibrio orientalis | ATGTAGGGAGCAGGGGACT | CACAGAACCTGTCACCGGT | 59.29 | 59.55 | 293 | 53 |
100.00%
|
100.00%
|
| Primer 1006 | Vibrio orientalis | AAGCAAACTGGCGTGACG | CACCCACTGAAGCCATGGT | 58.98 | 59.92 | 153 | 53 |
100.00%
|
100.00%
|
| Primer 1007 | Vibrio orientalis | TGCTGCTTGCCAATGGGT | AGCCAAGCGCAGGTAGAA | 60.20 | 58.93 | 289 | 53 |
100.00%
|
100.00%
|
| Primer 1008 | Vibrio orientalis | ATACGAGTCGCAGAGCACG | GATGATCGCATGGTGGGGT | 59.94 | 59.85 | 168 | 53 |
100.00%
|
100.00%
|
| Primer 1009 | Vibrio orientalis | AACAGCGCACTTCGTCGA | TCGCCAAACGCACCATGA | 59.97 | 60.28 | 217 | 53 |
100.00%
|
100.00%
|
| Primer 1010 | Vibrio orientalis | ACACAAGCTTGGTCGGCT | GCGACAACCAAATGGCCA | 59.49 | 58.95 | 187 | 53 |
100.00%
|
100.00%
|