| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Winkia neuii | TGGCGACAAGGGCAAACA | TTCCGGTCGCCTTGTTGT | 60.12 | 59.49 | 287 | 137 |
100.00%
|
100.00%
|
| Primer 2 | Winkia neuii | CGCAAACCGCAGCTTCAA | TGCCATCTGTGCTGCGAA | 59.67 | 59.97 | 196 | 128 |
100.00%
|
100.00%
|
| Primer 3 | Winkia neuii | ACGCGACTTGCTGGTGAA | CCCCAAAATCTGCGCAACC | 59.89 | 60.08 | 213 | 128 |
100.00%
|
100.00%
|
| Primer 4 | Winkia neuii | ACGCGACTTGCTGGTGAA | CACGCAAAACTTCGTCCCG | 59.89 | 60.08 | 163 | 128 |
100.00%
|
100.00%
|
| Primer 5 | Winkia neuii | TGGCGACAAGGGCAAACA | ATCCTTTCCGGTCGCCTTG | 60.12 | 60.08 | 292 | 126 |
100.00%
|
100.00%
|
| Primer 6 | Winkia neuii | TGGCGACAAGGGCAAACA | TTTCCGGTCGCCTTGTTGT | 60.12 | 60.15 | 288 | 126 |
100.00%
|
100.00%
|
| Primer 7 | Winkia neuii | TGGCGACAAGGGCAAACA | ACCTTGGCAGGCTTCTTGG | 60.12 | 60.23 | 210 | 126 |
100.00%
|
100.00%
|
| Primer 8 | Winkia neuii | TGGCGACAAGGGCAAACA | GGCAGGCTTCTTGGTGGAA | 60.12 | 60.23 | 205 | 117 |
100.00%
|
100.00%
|
| Primer 9 | Winkia neuii | TGGCGACAAGGGCAAACA | CCTTGGCAGGCTTCTTGGT | 60.12 | 60.23 | 209 | 117 |
100.00%
|
100.00%
|
| Primer 10 | Winkia neuii | GCGCAAGAACGTCACTCAC | TGCCATCTGTGCTGCGAA | 59.80 | 59.97 | 163 | 117 |
100.00%
|
100.00%
|