| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Xanthobacter autotrophicus | TGAGCCAACTCTTCCGCTG | TCGCCAGAAGCCAGTTCA | 60.00 | 58.85 | 181 | 65 |
100.00%
|
100.00%
|
| Primer 2 | Xanthobacter autotrophicus | GTGAGCCAACTCTTCCGCT | TCGCCAGAAGCCAGTTCA | 60.00 | 58.85 | 182 | 61 |
100.00%
|
100.00%
|
| Primer 3 | Xanthobacter autotrophicus | TCTGGTGAAGCCGTTCGAC | GGCTTCGACATGAGCTGGT | 60.01 | 60.08 | 222 | 61 |
100.00%
|
100.00%
|
| Primer 4 | Xanthobacter autotrophicus | TGAGCCAACTCTTCCGCTG | GTCGCCAGAAGCCAGTTCA | 60.00 | 60.30 | 182 | 59 |
100.00%
|
100.00%
|
| Primer 5 | Xanthobacter autotrophicus | GTGAGCCAACTCTTCCGCT | GTCGCCAGAAGCCAGTTCA | 60.00 | 60.30 | 183 | 55 |
100.00%
|
100.00%
|
| Primer 6 | Xanthobacter autotrophicus | TGAAGCCGTTCGACCTGT | GGCTTCGACATGAGCTGGT | 58.87 | 60.08 | 217 | 55 |
100.00%
|
100.00%
|