| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 131 | Acidithiobacillus | AACAAGCGCACCCTGGAA | AGGGACGTCGCAAGGTTT | 59.81 | 59.17 | 202 | 109 |
100.00%
|
50.00%
|
| Primer 132 | Acidithiobacillus | GCAGGTCCGGACTCCTTTT | AGCGTCCCTTTGGGCAAT | 59.62 | 59.56 | 275 | 109 |
100.00%
|
50.00%
|
| Primer 133 | Acidithiobacillus | CGGTATGACCTGCGGACAT | TTCCTTGACCGCTGCGAT | 59.56 | 59.34 | 151 | 108 |
100.00%
|
50.00%
|
| Primer 134 | Acidithiobacillus | TCCGCATAGCATCGCCAA | CGCTTCTCGAATCGCTCCA | 59.50 | 60.23 | 186 | 108 |
100.00%
|
50.00%
|
| Primer 135 | Acidithiobacillus | TCCGCATAGCATCGCCAA | TTCCAGGTTGGCGTTACCG | 59.50 | 60.30 | 250 | 108 |
100.00%
|
50.00%
|
| Primer 136 | Acidithiobacillus | TGCAGACCATTCGCCACA | AACAAGCCCTGACGTACGG | 59.57 | 60.01 | 232 | 108 |
100.00%
|
50.00%
|
| Primer 137 | Acidithiobacillus | TGCAGACCATTCGCCACA | ACCGAGGATGAGGGGACAA | 59.57 | 59.92 | 194 | 108 |
100.00%
|
50.00%
|
| Primer 138 | Acidithiobacillus | TGTCGAAGGTTGCTGCCA | TTGCCCTCGAAACCACCAA | 59.49 | 59.77 | 210 | 108 |
100.00%
|
50.00%
|
| Primer 139 | Acidithiobacillus | CCCCACTGATCGGCATCAA | CACGAGAAAGGCACCGACT | 59.78 | 60.01 | 176 | 108 |
100.00%
|
50.00%
|
| Primer 140 | Acidithiobacillus | ACAGGACATTGGACCGCTG | ACAACCCCAGTTCCCGAAC | 60.00 | 59.85 | 176 | 107 |
100.00%
|
50.00%
|