| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 281 | Acidithiobacillus | AACGATCTGCTGACCCTGC | AAAGCGTGCCGTCGAGAT | 60.08 | 59.74 | 226 | 85 |
100.00%
|
50.00%
|
| Primer 282 | Acidithiobacillus | TTTGCCATGCCTGCGGAT | ACAATGGAGCTGGACTGGC | 60.36 | 60.00 | 188 | 85 |
100.00%
|
50.00%
|
| Primer 283 | Acidithiobacillus | GGCTGCTGGACACCATCAT | TGCGGTTGATGAGCATGGT | 60.08 | 60.00 | 264 | 84 |
100.00%
|
50.00%
|
| Primer 284 | Acidithiobacillus | CGAGCATGTGGAGCCTGAA | ACACCACAAAAGCGCTGC | 60.08 | 59.59 | 191 | 84 |
100.00%
|
50.00%
|
| Primer 285 | Acidithiobacillus | ACGGAGCTGGCCAAAGAA | AGGCATTGGCACCGTTGA | 59.16 | 59.88 | 184 | 84 |
100.00%
|
50.00%
|
| Primer 286 | Acidithiobacillus | TGTGCGTTTCGTGCTGGA | AGAGCGGTGCAAACCAGT | 60.20 | 59.49 | 272 | 84 |
100.00%
|
50.00%
|
| Primer 287 | Acidithiobacillus | GCAACGTCGCCAGCAAAT | TGGCCTCGACAAACTCCAC | 59.74 | 59.93 | 279 | 84 |
100.00%
|
50.00%
|
| Primer 288 | Acidithiobacillus | GCAACGTCGCCAGCAAAT | GCCAGCATCTCGGGATTGA | 59.74 | 59.85 | 217 | 84 |
100.00%
|
50.00%
|
| Primer 289 | Acidithiobacillus | TGCGGTGGAATTGCGGAT | TGTCGTCTGGCACCGAAT | 60.05 | 58.94 | 236 | 84 |
100.00%
|
50.00%
|
| Primer 290 | Acidithiobacillus | TGCGGTGGAATTGCGGAT | ATGTCGTCTGGCACCGAA | 60.05 | 58.94 | 237 | 84 |
100.00%
|
50.00%
|