| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 51 | Acidithiobacillus | TGTCGAAGGTTGCTGCCA | TTGCCCTCGAAACCACCA | 59.49 | 59.08 | 210 | 124 |
100.00%
|
50.00%
|
| Primer 52 | Acidithiobacillus | CCTCTGCGAACCGGAATGT | ATCCTTGACGTGCAGCCA | 60.08 | 59.25 | 169 | 124 |
100.00%
|
50.00%
|
| Primer 53 | Acidithiobacillus | TCTGCGAACCGGAATGTCC | ATCCTTGACGTGCAGCCA | 60.08 | 59.25 | 167 | 124 |
100.00%
|
50.00%
|
| Primer 54 | Acidithiobacillus | CACGGTTTATGCCACAGCG | TCCCTCACCTCCGCATCTT | 59.87 | 60.31 | 231 | 124 |
100.00%
|
50.00%
|
| Primer 55 | Acidithiobacillus | TTGACGATGCGCTGACCA | TGGTCAAACGCGTTGTCG | 59.66 | 58.99 | 202 | 124 |
100.00%
|
50.00%
|
| Primer 56 | Acidithiobacillus | TTGACGATGCGCTGACCA | CTGGTCAAACGCGTTGTCG | 59.66 | 60.08 | 203 | 124 |
100.00%
|
50.00%
|
| Primer 57 | Acidithiobacillus | TGTACCCACCATCATGCCG | AACATCTGCGCTGCGGTA | 59.78 | 59.74 | 278 | 122 |
100.00%
|
50.00%
|
| Primer 58 | Acidithiobacillus | CACCATCATGCCGACCACA | AACATCTGCGCTGCGGTA | 60.38 | 59.74 | 272 | 122 |
100.00%
|
50.00%
|
| Primer 59 | Acidithiobacillus | ACCATCATGCCGACCACAC | AACATCTGCGCTGCGGTA | 60.38 | 59.74 | 271 | 122 |
100.00%
|
50.00%
|
| Primer 60 | Acidithiobacillus | TACCCACCATCATGCCGAC | AACATCTGCGCTGCGGTA | 59.48 | 59.74 | 276 | 122 |
100.00%
|
50.00%
|