| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Actinomyces | GGGTGTGGCTGATGCTCAT | TTTGAAGCTGGCAGGCCA | 60.08 | 59.80 | 286 | 135 |
100.00%
|
33.33%
|
| Primer 2 | Actinomyces | TAGTGGGTGTGGCTGATGC | TTTGAAGCTGGCAGGCCA | 59.70 | 59.80 | 290 | 135 |
100.00%
|
33.33%
|
| Primer 3 | Actinomyces | GTGTGGCTGATGCTCATGC | TTTGAAGCTGGCAGGCCA | 59.57 | 59.80 | 284 | 135 |
100.00%
|
33.33%
|
| Primer 4 | Actinomyces | TGACAGCCACAAACGCCA | ACGGATCTTGCTCAGCACC | 60.12 | 60.08 | 239 | 135 |
100.00%
|
33.33%
|
| Primer 5 | Actinomyces | TGACAGCCACAAACGCCA | CGACGGATCTTGCTCAGCA | 60.12 | 60.15 | 241 | 135 |
100.00%
|
33.33%
|
| Primer 6 | Actinomyces | TGACAGCCACAAACGCCA | TGCGACGGATCTTGCTCAG | 60.12 | 60.15 | 243 | 135 |
100.00%
|
33.33%
|
| Primer 7 | Actinomyces | TGACAGCCACAAACGCCA | TTGCGACGGATCTTGCTCA | 60.12 | 59.71 | 244 | 132 |
100.00%
|
33.33%
|
| Primer 8 | Actinomyces | TTGGCTTTGGCTGCTGGT | AAACCCCAATCACCTGCCC | 60.12 | 60.23 | 193 | 128 |
100.00%
|
33.33%
|
| Primer 9 | Actinomyces | TTGGCTTTGGCTGCTGGT | CTGCCTGCAAAACCCCAA | 60.12 | 58.45 | 202 | 128 |
100.00%
|
33.33%
|
| Primer 10 | Actinomyces | TTGGCTTTGGCTGCTGGT | AGCCCTAACGCTCTGACCA | 60.12 | 60.61 | 174 | 128 |
100.00%
|
33.33%
|