| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 4401 | Aeromonas | TGCGTGCACCCTGACTTT | GGACATCAGCACGGCATCT | 59.81 | 60.15 | 222 | 25 |
100.00%
|
37.50%
|
| Primer 4402 | Aeromonas | AAGGGGTGGTCTCCTTCGA | AAGGTGATCGCCTTGCCA | 59.84 | 59.24 | 157 | 25 |
100.00%
|
37.50%
|
| Primer 4403 | Aeromonas | TGAAGCAGCTCGATCGCA | TTGATGAAGGGCTCCTCGC | 59.43 | 59.78 | 286 | 25 |
100.00%
|
50.00%
|
| Primer 4404 | Aeromonas | TCGCGAACGACGGTGAAA | TTGTAACCACGGTGCAGGG | 59.97 | 60.23 | 157 | 25 |
100.00%
|
37.50%
|
| Primer 4405 | Aeromonas | CAGCCCACCATCAGCAAGA | AGAGCTCCACCTTGGGGTA | 60.00 | 59.53 | 280 | 25 |
100.00%
|
50.00%
|
| Primer 4406 | Aeromonas | ATCACGTGGCGTTTCGGA | GCACCTGATCCACCAGCTT | 59.66 | 60.00 | 240 | 25 |
100.00%
|
62.50%
|
| Primer 4407 | Aeromonas | CCTGCCTGCAGCTCTTCTT | AGACGATGGCCACGTTGT | 60.00 | 59.26 | 271 | 25 |
100.00%
|
75.00%
|
| Primer 4408 | Aeromonas | ATGGCTGCCATTTCCGGT | ACGGACGCGATCGACTTT | 59.64 | 59.44 | 282 | 25 |
100.00%
|
75.00%
|
| Primer 4409 | Aeromonas | AGCACAGCGTCAAAGGCT | ACAGACGGTAGCGCACTT | 59.89 | 58.95 | 204 | 25 |
100.00%
|
75.00%
|
| Primer 4410 | Aeromonas | GCCCTGTTCCTCGACAACA | TTCAGCCAGGCCTGATTCG | 59.93 | 60.08 | 170 | 24 |
100.00%
|
37.50%
|