| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 41 | Aeromonas | TTGCCGACTTCGACTGCA | TGACGATAACGCCAGCCA | 59.58 | 59.03 | 273 | 135 |
100.00%
|
62.50%
|
| Primer 42 | Aeromonas | TGGGACATCCTCGTCGAGT | ATTTGCCCACGTCGTCCA | 60.00 | 59.57 | 184 | 135 |
100.00%
|
87.50%
|
| Primer 43 | Aeromonas | TCGACCTGCGACTACAAGC | ATTTGCCCACGTCGTCCA | 59.79 | 59.57 | 205 | 135 |
100.00%
|
87.50%
|
| Primer 44 | Aeromonas | GCGACTACAAGCTGTGGGA | ATTTGCCCACGTCGTCCA | 59.71 | 59.57 | 198 | 135 |
100.00%
|
87.50%
|
| Primer 45 | Aeromonas | AGCCGACATTGCAGAGCA | TTTCGACCCTGGCCTTGA | 59.65 | 58.43 | 263 | 132 |
100.00%
|
87.50%
|
| Primer 46 | Aeromonas | AGCCGACATTGCAGAGCA | CCTGGCCTTGAGCTTCTGA | 59.65 | 59.32 | 256 | 132 |
100.00%
|
87.50%
|
| Primer 47 | Aeromonas | TGGGCTTTGTCGCGTTCA | ACAAACAGCACCACGACCA | 60.20 | 60.08 | 205 | 132 |
100.00%
|
50.00%
|
| Primer 48 | Aeromonas | TGGGCTTTGTCGCGTTCA | CACGACCAGGGCAAATTGC | 60.20 | 60.08 | 194 | 132 |
100.00%
|
37.50%
|
| Primer 49 | Aeromonas | TGGGCTTTGTCGCGTTCA | ACGACCAGGGCAAATTGC | 60.20 | 58.63 | 193 | 132 |
100.00%
|
37.50%
|
| Primer 50 | Aeromonas | TTGCCGACTTCGACTGCA | GAACCCTGGAAACGGCTCA | 59.58 | 59.93 | 157 | 132 |
100.00%
|
62.50%
|