| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Afipia | ATGCACAATCACCCCTCCG | TCAGCCTCATGCCCTTGAG | 60.08 | 59.39 | 157 | 104 |
100.00%
|
66.67%
|
| Primer 2 | Afipia | ATGCACAATCACCCCTCCG | TCAGCCTCATGCCCTTGA | 60.08 | 58.18 | 157 | 101 |
100.00%
|
66.67%
|
| Primer 3 | Afipia | ACGTCGATTGCGGTGACA | GAATCATGCGCTCGATGGC | 59.66 | 59.79 | 192 | 99 |
100.00%
|
66.67%
|
| Primer 4 | Afipia | TCACACTCGCGTTCGACAT | TCGCCAAACGCATGACCA | 59.72 | 60.28 | 298 | 97 |
100.00%
|
66.67%
|
| Primer 5 | Afipia | ATGCACAATCACCCCTCCG | ACATCAGCCTCATGCCCTT | 60.08 | 58.99 | 160 | 94 |
100.00%
|
66.67%
|
| Primer 6 | Afipia | ACACTCGCGTTCGACATCA | TCGCCAAACGCATGACCA | 59.72 | 60.28 | 296 | 94 |
100.00%
|
66.67%
|
| Primer 7 | Afipia | ATGCACAATCACCCCTCCG | ACATCAGCCTCATGCCCT | 60.08 | 58.25 | 160 | 91 |
100.00%
|
66.67%
|
| Primer 8 | Afipia | ACCGTTTCGCCAAGGATCA | ATGGTTTCCTGCTCCTCGC | 59.63 | 60.08 | 196 | 87 |
100.00%
|
66.67%
|
| Primer 9 | Afipia | CGTTTCGCCAAGGATCAGC | ATGGTTTCCTGCTCCTCGC | 59.57 | 60.08 | 194 | 87 |
100.00%
|
66.67%
|
| Primer 10 | Afipia | CCACCGTTTCGCCAAGGAT | ATGGTTTCCTGCTCCTCGC | 60.67 | 60.08 | 198 | 87 |
100.00%
|
66.67%
|