| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Aggregatibacter | CGTCAGGTGAGTGCAGTGT | ACCGGAATGCGCAAATGC | 59.93 | 59.82 | 155 | 148 |
100.00%
|
100.00%
|
| Primer 2 | Aggregatibacter | CGGTGTGACGGTGGAAGAT | ACCGGAATGCGCAAATGC | 59.71 | 59.82 | 189 | 141 |
100.00%
|
50.00%
|
| Primer 3 | Aggregatibacter | TGACTGCCGTTCAAGCCAT | TGACTGCGTTCGCCATGT | 59.93 | 59.97 | 258 | 137 |
100.00%
|
50.00%
|
| Primer 4 | Aggregatibacter | CGTAGTGTGCAAGGTCGTG | CGACATTCGCGAACTTCCAC | 58.86 | 59.91 | 179 | 136 |
100.00%
|
100.00%
|
| Primer 5 | Aggregatibacter | TCGTAGTGTGCAAGGTCGTG | CGACATTCGCGAACTTCCAC | 60.32 | 59.91 | 180 | 136 |
100.00%
|
100.00%
|
| Primer 6 | Aggregatibacter | TGCCAAGCAGAAGAAGCCA | AGCCATTTTCCACCGCACT | 59.85 | 60.23 | 205 | 135 |
100.00%
|
50.00%
|
| Primer 7 | Aggregatibacter | ACAAGGTTGCGTTGCAGC | CCAAACTGCTGGCGACTTC | 59.59 | 59.42 | 248 | 129 |
100.00%
|
75.00%
|
| Primer 8 | Aggregatibacter | AATGCCAGCGTGTCGGT | ACCGGAATGCGCAAATGC | 59.60 | 59.82 | 244 | 128 |
100.00%
|
50.00%
|
| Primer 9 | Aggregatibacter | CACTGTTGAGGCGTTTGGC | ACGCGTGAACCGGAAGAA | 60.01 | 59.58 | 228 | 128 |
100.00%
|
50.00%
|
| Primer 10 | Aggregatibacter | CGTTTGGCAATCCGGTGAC | ACGCGTGAACCGGAAGAA | 59.79 | 59.58 | 217 | 128 |
100.00%
|
50.00%
|