| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Akkermansia | TTTTGCGTGTTGGGCAGC | GTGCACGCCTTTCATGTGG | 59.89 | 60.08 | 192 | 154 |
100.00%
|
100.00%
|
| Primer 2 | Akkermansia | ACGCTGGATGCCAATGACA | ACGCGTTCCAGCTCAACA | 60.00 | 59.89 | 284 | 137 |
100.00%
|
40.00%
|
| Primer 3 | Akkermansia | AGGACCCCAATTACGCTGC | ACGCGTTCCAGCTCAACA | 60.08 | 59.89 | 184 | 137 |
100.00%
|
20.00%
|
| Primer 4 | Akkermansia | ACCTAAGCGCAGAACGTCC | ACGCTTCCGCTTTCACCA | 60.08 | 59.89 | 182 | 135 |
100.00%
|
100.00%
|
| Primer 5 | Akkermansia | CCTAAGCGCAGAACGTCCA | ACGCTTCCGCTTTCACCA | 60.08 | 59.89 | 181 | 135 |
100.00%
|
100.00%
|
| Primer 6 | Akkermansia | AGATGAAGCAGCGTACCCG | ACGCTTCCGCTTTCACCA | 59.86 | 59.89 | 162 | 135 |
100.00%
|
100.00%
|
| Primer 7 | Akkermansia | AGCGCAGAACGTCCAAGAT | ACGCTTCCGCTTTCACCA | 59.71 | 59.89 | 177 | 135 |
100.00%
|
100.00%
|
| Primer 8 | Akkermansia | GCGCAGAACGTCCAAGATG | ACGCTTCCGCTTTCACCA | 59.58 | 59.89 | 176 | 135 |
100.00%
|
100.00%
|
| Primer 9 | Akkermansia | ACATGCACATCGACGGCA | TTGAGTTCGCAGCGGTCA | 60.05 | 59.58 | 160 | 132 |
100.00%
|
100.00%
|
| Primer 10 | Akkermansia | CGTGCGCAATTGAAGGCT | GGCCAGTTCACGGAAGGAA | 59.44 | 59.93 | 159 | 128 |
100.00%
|
100.00%
|