| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 31 | Akkermansia | CGCCAGTGGTTTGCTCATG | AGCTTCGTTGTGCTCCGT | 59.79 | 59.58 | 165 | 112 |
100.00%
|
60.00%
|
| Primer 32 | Akkermansia | ACATAGCTCCATCCACGGC | AGCTTCGTTGTGCTCCGT | 59.55 | 59.58 | 188 | 112 |
100.00%
|
60.00%
|
| Primer 33 | Akkermansia | CGCCAGTGGTTTGCTCAT | AGCTTCGTTGTGCTCCGT | 58.33 | 59.58 | 165 | 112 |
100.00%
|
60.00%
|
| Primer 34 | Akkermansia | TTTTGCGTGTTGGGCAGC | ACTGGTGCACGCCTTTCA | 59.89 | 59.81 | 196 | 110 |
100.00%
|
80.00%
|
| Primer 35 | Akkermansia | TTTTGCGTGTTGGGCAGC | ACGGCATGCTTTCTGGTGA | 59.89 | 59.93 | 215 | 110 |
100.00%
|
60.00%
|
| Primer 36 | Akkermansia | TGTGCGACGCCATCAACA | CCGTCTTCATCCAGGGCAA | 60.28 | 59.70 | 229 | 108 |
100.00%
|
60.00%
|
| Primer 37 | Akkermansia | CCAGTCCCAGAACGTGCAT | AGGCACGGAAAATGCGGT | 60.00 | 60.28 | 203 | 106 |
100.00%
|
40.00%
|
| Primer 38 | Akkermansia | AACGACCGCAGCAGCAAA | TTCGCCTGCTTCGTGGAA | 60.90 | 59.58 | 214 | 106 |
100.00%
|
20.00%
|
| Primer 39 | Akkermansia | ACGACCAAGAAGCACGCT | GGCCTTGAAAGCAACCGTC | 59.58 | 59.71 | 198 | 106 |
100.00%
|
80.00%
|
| Primer 40 | Akkermansia | ACGACCAAGAAGCACGCT | GAGGCCTTGAAAGCAACCG | 59.58 | 59.42 | 200 | 106 |
100.00%
|
80.00%
|