| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1001 | Alkalihalobacillus | GCCTTTCTAAGCGCAGGGA | GCAGATCGCCTCCAAACAC | 60.08 | 59.20 | 169 | 86 |
100.00%
|
50.00%
|
| Primer 1002 | Alkalihalobacillus | GCCTTTCTAAGCGCAGGGA | TACCCAAGCAGATCGCCTC | 60.08 | 59.18 | 176 | 86 |
100.00%
|
50.00%
|
| Primer 1003 | Alkalihalobacillus | AGAATCGGTGCGAGTTGC | CAACGACGCGAACTTGCAA | 58.12 | 60.01 | 224 | 85 |
100.00%
|
50.00%
|
| Primer 1004 | Alkalihalobacillus | GAAACGCTGGTGCCACAA | ACCCCAATCGTTGTGGCA | 58.88 | 59.48 | 232 | 85 |
100.00%
|
50.00%
|
| Primer 1005 | Alkalihalobacillus | GGCTATGGATGATGGTGGGA | ATACAAGGCTGGCCACGAC | 58.93 | 60.08 | 155 | 85 |
100.00%
|
50.00%
|
| Primer 1006 | Alkalihalobacillus | GACTTTTGCTGGGGACGGA | TCGCACTCCCTGTTGCAA | 59.93 | 59.49 | 188 | 85 |
100.00%
|
50.00%
|
| Primer 1007 | Alkalihalobacillus | AGCTGTCGGGATGGTTTGG | GCAAAGGGTCCAGCTCCAT | 60.00 | 60.00 | 246 | 85 |
100.00%
|
50.00%
|
| Primer 1008 | Alkalihalobacillus | TGGTGAACCAGGTGGAGGA | ACCTCACTGCCGAAACCAG | 60.07 | 59.93 | 231 | 85 |
100.00%
|
50.00%
|
| Primer 1009 | Alkalihalobacillus | GCATCGCCTGAACTAGTGG | CCCGTTCCTTGAAGTACCCC | 58.32 | 60.04 | 158 | 85 |
100.00%
|
50.00%
|
| Primer 1010 | Alkalihalobacillus | GCATCGCCTGAACTAGTGGA | TCCCGTTCCTTGAAGTACCC | 59.82 | 59.02 | 159 | 85 |
100.00%
|
50.00%
|