| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 4521 | Alkalihalobacillus | AACTTCAGGCGACGTCGA | GTCGTCGCGATTTGAACGG | 58.96 | 59.94 | 285 | 54 |
100.00%
|
50.00%
|
| Primer 4522 | Alkalihalobacillus | AACTTCAGGCGACGTCGA | TCGTCGCGATTTGAACGGA | 58.96 | 60.08 | 284 | 54 |
100.00%
|
50.00%
|
| Primer 4523 | Alkalihalobacillus | GCCGATGATCGATGGTCGT | TCGTCCCCATCACTTCCGA | 60.01 | 60.30 | 206 | 54 |
100.00%
|
50.00%
|
| Primer 4524 | Alkalihalobacillus | TGCCGATGATCGATGGTCG | TCGTCCCCATCACTTCCGA | 60.01 | 60.30 | 207 | 54 |
100.00%
|
50.00%
|
| Primer 4525 | Alkalihalobacillus | TGATCGTTCGGACAGGGGA | CGGCATTACGGACACTCGA | 60.30 | 59.86 | 176 | 54 |
100.00%
|
50.00%
|
| Primer 4526 | Alkalihalobacillus | TTGCCAGCGGAAAGTCGA | TTCACCGAGACGCTTTCGA | 59.58 | 59.34 | 185 | 54 |
100.00%
|
50.00%
|
| Primer 4527 | Alkalihalobacillus | TTGCCAGCGGAAAGTCGA | ACCGAGACGCTTTCGATGA | 59.58 | 59.12 | 182 | 54 |
100.00%
|
50.00%
|
| Primer 4528 | Alkalihalobacillus | TTGCCAGCGGAAAGTCGA | TCACCGAGACGCTTTCGAT | 59.58 | 59.12 | 184 | 54 |
100.00%
|
50.00%
|
| Primer 4529 | Alkalihalobacillus | TTGCCAGCGGAAAGTCGA | CACCGAGACGCTTTCGATG | 59.58 | 59.01 | 183 | 54 |
100.00%
|
50.00%
|
| Primer 4530 | Alkalihalobacillus | TTGCCAGCGGAAAGTCGA | TTTCACCGAGACGCTTTCGA | 59.58 | 59.97 | 186 | 54 |
100.00%
|
50.00%
|