| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 5081 | Alkalihalobacillus | AACACGCTTTGCTCGTCCT | GCGCTCATCAACGACACA | 60.23 | 58.45 | 266 | 49 |
100.00%
|
50.00%
|
| Primer 5082 | Alkalihalobacillus | GCGGAGGGCAAAGATGAAC | TCGCCTTCCTGCAATCCT | 59.20 | 58.60 | 188 | 49 |
100.00%
|
50.00%
|
| Primer 5083 | Alkalihalobacillus | AACTGGCAATGGGGTTGTGA | CGTTCAGACCCAACCGCTA | 60.11 | 59.71 | 153 | 49 |
100.00%
|
50.00%
|
| Primer 5084 | Alkalihalobacillus | ATGGGTGCTTTGGTCGCT | GCTCGAACCGCATCTGCTA | 59.56 | 60.23 | 275 | 49 |
100.00%
|
50.00%
|
| Primer 5085 | Alkalihalobacillus | GCTGTTTGCCCTGGGAGTT | ACCACCAAACGAGTCCACC | 60.53 | 59.85 | 204 | 49 |
100.00%
|
50.00%
|
| Primer 5086 | Alkalihalobacillus | ATGTCCCTGTTGCCCATGG | ACGTTCTGGGTGTGGCAT | 60.00 | 59.16 | 182 | 49 |
100.00%
|
50.00%
|
| Primer 5087 | Alkalihalobacillus | GGTCATTTACACGCTGTACCT | TCGTGCCAATCCGCAAGT | 58.03 | 59.97 | 251 | 49 |
100.00%
|
50.00%
|
| Primer 5088 | Alkalihalobacillus | TGAGGCCTTTTGAGGGTGTT | TGCCATCCAGCGTTCCTT | 59.44 | 59.24 | 298 | 49 |
100.00%
|
50.00%
|
| Primer 5089 | Alkalihalobacillus | TGAGGCCTTTTGAGGGTGTT | TTGCCATCCAGCGTTCCT | 59.44 | 59.24 | 299 | 49 |
100.00%
|
50.00%
|
| Primer 5090 | Alkalihalobacillus | TGAGGCCTTTTGAGGGTGTT | TGTGACCACGCATCACCA | 59.44 | 59.17 | 265 | 49 |
100.00%
|
50.00%
|