| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 5721 | Alkalihalobacillus | ACGGTCATTGCAGGAGGA | AAAAGCCGTCGCAATCGC | 58.19 | 59.82 | 288 | 43 |
100.00%
|
50.00%
|
| Primer 5722 | Alkalihalobacillus | CGGAGGCGAGCCAAAGAAT | CAAAAGGAAAGGTCGAATCGC | 60.45 | 58.15 | 152 | 43 |
100.00%
|
50.00%
|
| Primer 5723 | Alkalihalobacillus | ACCGTACTGGACAGGCAGA | CCTGTCGGCTACGTCTAAGC | 60.23 | 60.25 | 288 | 43 |
100.00%
|
50.00%
|
| Primer 5724 | Alkalihalobacillus | AGGCTGCTGTGAACGCTT | TCCTTCACGCCATACCACC | 59.89 | 59.40 | 283 | 43 |
100.00%
|
50.00%
|
| Primer 5725 | Alkalihalobacillus | AGGCTGCTGTGAACGCTT | CGCCATACCACCTCAATGC | 59.89 | 58.98 | 276 | 43 |
100.00%
|
50.00%
|
| Primer 5726 | Alkalihalobacillus | GGGGAAGGCTCATCAAACCA | ACGCACGTTCTCTCGCTT | 59.96 | 59.66 | 244 | 43 |
100.00%
|
50.00%
|
| Primer 5727 | Alkalihalobacillus | AGCTCATGTCAGCGATGCA | TTGTTGCCCAAGTGTCGC | 59.78 | 58.88 | 263 | 43 |
100.00%
|
50.00%
|
| Primer 5728 | Alkalihalobacillus | TGTGGCCACGCTTGAACT | CTAAAACGGCAGCCATCGC | 59.81 | 59.94 | 197 | 43 |
100.00%
|
50.00%
|
| Primer 5729 | Alkalihalobacillus | TGTGGCCACGCTTGAACT | TAAAACGGCAGCCATCGC | 59.81 | 58.82 | 196 | 43 |
100.00%
|
50.00%
|
| Primer 5730 | Alkalihalobacillus | TTTCCAGGTCAAGGCTCGC | TTCAAGCGTGGCCACACT | 60.30 | 59.81 | 204 | 43 |
100.00%
|
50.00%
|