| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 6141 | Alkalihalobacillus | TGAAGAAACCGAGCCCACC | TTCGACCACAACACCAGCC | 59.93 | 60.53 | 242 | 38 |
100.00%
|
50.00%
|
| Primer 6142 | Alkalihalobacillus | ACTGCGCTTCTTAATGGCAA | ACAACCAGACACCGTCTCA | 58.47 | 58.49 | 156 | 38 |
100.00%
|
50.00%
|
| Primer 6143 | Alkalihalobacillus | TGCTGACCGTGTGCATGT | TCTTCGTTTCCGCTGGCA | 59.89 | 59.58 | 260 | 38 |
100.00%
|
50.00%
|
| Primer 6144 | Alkalihalobacillus | TGCTACTTGCGGTCATGGG | TGGCAGCCGAATCGCTAAT | 60.08 | 59.85 | 203 | 38 |
100.00%
|
50.00%
|
| Primer 6145 | Alkalihalobacillus | AGGCAAAGCACGGCTCAA | TGCTGCCAGCGACAACAT | 60.20 | 60.28 | 170 | 38 |
100.00%
|
50.00%
|
| Primer 6146 | Alkalihalobacillus | AGGCAAAGCACGGCTCAA | ATGCTGCCAGCGACAACA | 60.20 | 60.28 | 171 | 38 |
100.00%
|
50.00%
|
| Primer 6147 | Alkalihalobacillus | TGCTTCCATCGGCAGCAA | CCCACTTGCCACCATTTGC | 59.97 | 60.00 | 199 | 38 |
100.00%
|
50.00%
|
| Primer 6148 | Alkalihalobacillus | TCTGCGCTGCGAGAACAA | ATAAGCGTGCCTTCTCGGG | 59.97 | 59.86 | 215 | 38 |
100.00%
|
50.00%
|
| Primer 6149 | Alkalihalobacillus | ACGTGCGTGGATCGTTGA | AGCGTATCAAGGCGATCGG | 59.66 | 60.01 | 204 | 38 |
100.00%
|
50.00%
|
| Primer 6150 | Alkalihalobacillus | ATCCGAGCAACACAAGGGG | TTTGCACGCCTTCCCCTT | 60.00 | 59.80 | 193 | 38 |
100.00%
|
50.00%
|