| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 131 | Amedibacillus | GTCCCAAATGTGGCTGTCATG | CGCTATCGGTTTCTTCGCG | 59.80 | 59.45 | 156 | 99 |
100.00%
|
50.00%
|
| Primer 132 | Amedibacillus | TCTGCCGACAAGCTTAGCG | AGAACTGTCTGCATGCGCT | 60.45 | 60.00 | 299 | 99 |
100.00%
|
50.00%
|
| Primer 133 | Amedibacillus | GAGGGAGATGTGCGTTTCG | ACCAACAATCTCGGCTGCT | 58.63 | 59.62 | 154 | 99 |
100.00%
|
50.00%
|
| Primer 134 | Amedibacillus | TGAGGGAGATGTGCGTTTCG | ACCAACAATCTCGGCTGCT | 60.39 | 59.62 | 155 | 99 |
100.00%
|
50.00%
|
| Primer 135 | Amedibacillus | TCGAAAGCTTGCTCGGTGT | GCATTGCACCGCTGATGT | 59.93 | 59.12 | 249 | 98 |
100.00%
|
100.00%
|
| Primer 136 | Amedibacillus | CGTGTAGGGTGGGCAATGT | GGTCTTAGGCGACTCGTAGC | 60.00 | 59.97 | 263 | 98 |
100.00%
|
50.00%
|
| Primer 137 | Amedibacillus | ATGCTGAGACGTGCGCAT | TGCCCATGCAATGCTGGA | 60.13 | 59.96 | 195 | 98 |
100.00%
|
50.00%
|
| Primer 138 | Amedibacillus | ACGTGCGCATGTGGAGAT | TGCCCATGCAATGCTGGA | 59.73 | 59.96 | 187 | 98 |
100.00%
|
50.00%
|
| Primer 139 | Amedibacillus | CGTGCGCATGTGGAGATTG | TGCCCATGCAATGCTGGA | 59.94 | 59.96 | 186 | 98 |
100.00%
|
50.00%
|
| Primer 140 | Amedibacillus | TGCTGAGACGTGCGCAT | TGCCCATGCAATGCTGGA | 59.69 | 59.96 | 194 | 98 |
100.00%
|
50.00%
|