| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 11 | Amedibacillus | TTGCGGATTTGCATGGAGC | GGCTGTGATCGAAGCAAAGG | 59.49 | 59.55 | 261 | 118 |
100.00%
|
50.00%
|
| Primer 12 | Amedibacillus | ATTGCGGATTTGCATGGAGC | GGCTGTGATCGAAGCAAAGG | 59.90 | 59.55 | 262 | 118 |
100.00%
|
50.00%
|
| Primer 13 | Amedibacillus | CGGCATGGATCGGCTATGT | TCCATGACCTGCCCAATGG | 60.00 | 59.69 | 179 | 115 |
100.00%
|
100.00%
|
| Primer 14 | Amedibacillus | TTCAGACCTGTGCGGAGGA | CGTAGCCAAGCAATCGCAC | 60.53 | 59.94 | 152 | 115 |
100.00%
|
50.00%
|
| Primer 15 | Amedibacillus | CCGGTGGACAAAAGCAACG | AGCCTCTTCTGGATCGTGC | 60.01 | 59.48 | 188 | 115 |
100.00%
|
50.00%
|
| Primer 16 | Amedibacillus | AGCGTGGCATCAAGAAGGA | TCAACTCACGTTCCGCTCC | 59.32 | 60.01 | 185 | 115 |
100.00%
|
50.00%
|
| Primer 17 | Amedibacillus | ACGGTTGAACGAGTCGCA | GCACGAATCCCCAAAGCAC | 59.59 | 59.79 | 251 | 113 |
100.00%
|
100.00%
|
| Primer 18 | Amedibacillus | ACGGTTGAACGAGTCGCA | TGCACGAATCCCCAAAGCA | 59.59 | 60.23 | 252 | 113 |
100.00%
|
100.00%
|
| Primer 19 | Amedibacillus | ACGGTTGAACGAGTCGCA | CTGCACGAATCCCCAAAGC | 59.59 | 59.49 | 253 | 113 |
100.00%
|
100.00%
|
| Primer 20 | Amedibacillus | ACGGTTGAACGAGTCGCA | GCACGAATCCCCAAAGCA | 59.59 | 58.32 | 251 | 113 |
100.00%
|
100.00%
|