| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 51 | Amedibacillus | ACGCTGCTCGCCAAATGA | TGCATTGCAGCCTTCCCA | 60.36 | 59.88 | 224 | 107 |
100.00%
|
50.00%
|
| Primer 52 | Amedibacillus | TCGGTTTGATGATTGCGGTG | AAAGTCCAGCCACCGTAGC | 59.20 | 60.00 | 243 | 106 |
100.00%
|
50.00%
|
| Primer 53 | Amedibacillus | ACGGTTGAACGAGTCGCA | TCCTGCACGAATCCCCAA | 59.59 | 58.51 | 255 | 105 |
100.00%
|
100.00%
|
| Primer 54 | Amedibacillus | GTGAGCGTGCGCAAAACT | TCCGTTGCGCACCTTGAT | 59.67 | 59.97 | 257 | 105 |
100.00%
|
100.00%
|
| Primer 55 | Amedibacillus | ATGGCTGCGAATTATAGCGAT | GGCTTTGGAGATGTTTGCGG | 58.02 | 60.11 | 234 | 105 |
100.00%
|
100.00%
|
| Primer 56 | Amedibacillus | TCGGACAACGTTCGATTTCAA | ATGATCGGGAAGACTGCGG | 58.53 | 59.56 | 197 | 105 |
100.00%
|
50.00%
|
| Primer 57 | Amedibacillus | TCGGACAACGTTCGATTTCAA | CGGGAAGACTGCGGTACAT | 58.53 | 59.48 | 192 | 105 |
100.00%
|
50.00%
|
| Primer 58 | Amedibacillus | TCGGACAACGTTCGATTTCAA | TCGGGAAGACTGCGGTACA | 58.53 | 60.60 | 193 | 105 |
100.00%
|
50.00%
|
| Primer 59 | Amedibacillus | TCGGACAACGTTCGATTTCAA | ATCGGGAAGACTGCGGTAC | 58.53 | 59.19 | 194 | 105 |
100.00%
|
50.00%
|
| Primer 60 | Amedibacillus | TCGGACAACGTTCGATTTCAA | GATCGGGAAGACTGCGGTA | 58.53 | 58.89 | 195 | 105 |
100.00%
|
50.00%
|