| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 41 | Anaerobutyricum | CGATTTGTGAGGGCTGGAGA | GGAGCTTCCTCGCTGCAAT | 59.75 | 60.45 | 160 | 125 |
100.00%
|
66.67%
|
| Primer 42 | Anaerobutyricum | GCCCAGCGTCTTTCCTGAT | ACTTCCTCATCGGTTCCGC | 60.08 | 59.78 | 166 | 125 |
100.00%
|
33.33%
|
| Primer 43 | Anaerobutyricum | GCCGTTGGAATCAGTCGGA | TTGCCTTGCAGTCTGCCA | 60.08 | 59.80 | 160 | 124 |
100.00%
|
33.33%
|
| Primer 44 | Anaerobutyricum | CCATGCAAAAGGAGCCTGC | AGTTCCGCTGCCTTTTGC | 59.78 | 58.96 | 226 | 124 |
100.00%
|
66.67%
|
| Primer 45 | Anaerobutyricum | GCCATGCAAAAGGAGCCTG | AGTTCCGCTGCCTTTTGC | 59.78 | 58.96 | 227 | 124 |
100.00%
|
66.67%
|
| Primer 46 | Anaerobutyricum | CCTGCCATGCAAAAGGAGC | AGTTCCGCTGCCTTTTGC | 59.78 | 58.96 | 230 | 124 |
100.00%
|
66.67%
|
| Primer 47 | Anaerobutyricum | AGCAAATCTCGCACAGGGA | ACCGCTGACAGCAAGGTT | 59.32 | 59.49 | 264 | 122 |
100.00%
|
33.33%
|
| Primer 48 | Anaerobutyricum | CGGGAGCAAATCTCGCACA | ACCGCTGACAGCAAGGTT | 60.74 | 59.49 | 268 | 122 |
100.00%
|
33.33%
|
| Primer 49 | Anaerobutyricum | GCTGCTGCCACTTGAGTT | ACCGCTGACAGCAAGGTT | 58.24 | 59.49 | 153 | 122 |
100.00%
|
33.33%
|
| Primer 50 | Anaerobutyricum | AGCAAATCTCGCACAGGGAA | ACCGCTGACAGCAAGGTT | 59.96 | 59.49 | 264 | 122 |
100.00%
|
33.33%
|