| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Anaerocolumna | TCCATTATCGGTCCATGCCTG | CGGCTTTCACTGCTTCTGC | 59.93 | 59.79 | 270 | 157 |
100.00%
|
100.00%
|
| Primer 2 | Anaerocolumna | AGATGGCTGCAATCCCCTG | AGGATAACCGGTGCCATGG | 59.77 | 59.47 | 252 | 152 |
100.00%
|
100.00%
|
| Primer 3 | Anaerocolumna | GGCAACATTGTGTACATCTGC | ACACTCACCCCATTGCCAT | 58.13 | 59.22 | 169 | 149 |
100.00%
|
100.00%
|
| Primer 4 | Anaerocolumna | GGCAACATTGTGTACATCTGC | AACACTCACCCCATTGCCAT | 58.13 | 59.89 | 170 | 149 |
100.00%
|
100.00%
|
| Primer 5 | Anaerocolumna | TGGCTCAAAGGTATGGCCAT | CGGCTGACGGGTTTTGTTG | 59.37 | 60.01 | 202 | 149 |
100.00%
|
100.00%
|
| Primer 6 | Anaerocolumna | TGGCAATTCCGGTGGCAT | TCCGTTTAGCCCTGCTAGC | 59.96 | 59.48 | 203 | 149 |
100.00%
|
100.00%
|
| Primer 7 | Anaerocolumna | TGCAACCGTCCGTGTTGT | CCCTATGGAAAACGCCAATGC | 60.13 | 60.20 | 166 | 148 |
100.00%
|
100.00%
|
| Primer 8 | Anaerocolumna | TGCAACCGTCCGTGTTGT | GGAAAACGCCAATGCAAACAC | 60.13 | 59.74 | 160 | 148 |
100.00%
|
100.00%
|
| Primer 9 | Anaerocolumna | TGCAACCGTCCGTGTTGT | TGGAAAACGCCAATGCAAAC | 60.13 | 58.42 | 161 | 148 |
100.00%
|
100.00%
|
| Primer 10 | Anaerocolumna | TGACCCCAGAAAGTTGGCAT | TGCCTCTGGTAAGTCTCTTGT | 59.52 | 58.39 | 181 | 148 |
100.00%
|
100.00%
|