| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1001 | Anaerocolumna | TGCAAAAGCCATTCGCGA | TCCAGGGAAGCTGATGCAC | 58.65 | 59.70 | 162 | 87 |
100.00%
|
100.00%
|
| Primer 1002 | Anaerocolumna | CAGCTAAACCGGACTGCGA | ACAGGCCTGTGTAAGTGAGC | 60.08 | 59.96 | 276 | 87 |
100.00%
|
100.00%
|
| Primer 1003 | Anaerocolumna | AATGCTCCAGACAGGTGGC | TTCCCCAGTAAGCTGTGGC | 60.00 | 59.62 | 193 | 87 |
100.00%
|
100.00%
|
| Primer 1004 | Anaerocolumna | GCATGCCCTGACTCACAGT | TACCTCCGGTTGCTGCTTC | 60.00 | 59.70 | 266 | 87 |
100.00%
|
100.00%
|
| Primer 1005 | Anaerocolumna | AGAAGCGAAACGTGCCGA | GCAGACTTCAAATCCCTTGGG | 59.97 | 59.18 | 202 | 87 |
100.00%
|
100.00%
|
| Primer 1006 | Anaerocolumna | AGAAGCGAAACGTGCCGA | TGCAGACTTCAAATCCCTTGG | 59.97 | 58.48 | 203 | 87 |
100.00%
|
100.00%
|
| Primer 1007 | Anaerocolumna | AGAAGCGAAACGTGCCGA | CTGCACGGTTAGATAACAGCA | 59.97 | 58.38 | 269 | 87 |
100.00%
|
100.00%
|
| Primer 1008 | Anaerocolumna | AGAAGCGAAACGTGCCGA | CAGCTGCACGGTTAGATAACA | 59.97 | 58.38 | 272 | 87 |
100.00%
|
100.00%
|
| Primer 1009 | Anaerocolumna | AGCAAGGGTGGCAGGAAT | CGCTTTTGCCTCCGGTTT | 58.82 | 58.96 | 287 | 87 |
100.00%
|
100.00%
|
| Primer 1010 | Anaerocolumna | GTAGCAAGGGTGGCAGGAA | CGCTTTTGCCTCCGGTTT | 59.62 | 58.96 | 289 | 87 |
100.00%
|
100.00%
|