| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1521 | Anaerocolumna | GGCAATCGGAGGGCATGTA | CGGCAGTTCTCCATCCACA | 59.85 | 59.70 | 235 | 77 |
100.00%
|
100.00%
|
| Primer 1522 | Anaerocolumna | CGGTCACTTCCGGATGATG | TGCCTGGCAGGAAAGGTA | 58.02 | 58.09 | 190 | 77 |
100.00%
|
100.00%
|
| Primer 1523 | Anaerocolumna | AAAGCCCTGCCGTTCTTCC | CTTGCACGCTCTACCCGTA | 60.60 | 59.49 | 279 | 77 |
100.00%
|
100.00%
|
| Primer 1524 | Anaerocolumna | AAAGCCCTGCCGTTCTTCC | TTGCACGCTCTACCCGTA | 60.60 | 58.32 | 278 | 77 |
100.00%
|
100.00%
|
| Primer 1525 | Anaerocolumna | GCACTTACTGGCTGCGGTA | TTGAGCGGAAGGTGTTGCA | 60.08 | 60.15 | 255 | 77 |
100.00%
|
100.00%
|
| Primer 1526 | Anaerocolumna | GCAGCGGTAGGGCTTAGTA | TTCCTCCCTGCCGTCTTTG | 58.88 | 59.63 | 202 | 77 |
100.00%
|
100.00%
|
| Primer 1527 | Anaerocolumna | GTGGTCAGCCCAGGGTTTT | CGGGAAGGATCTCTACCGGA | 60.15 | 60.18 | 181 | 76 |
100.00%
|
100.00%
|
| Primer 1528 | Anaerocolumna | CATGCCGAACTGGACGTCT | ACAAAGAGGCACCAGCGA | 60.08 | 59.17 | 263 | 76 |
100.00%
|
100.00%
|
| Primer 1529 | Anaerocolumna | CACCTGTCCGCTTGTGGTA | TGGCACGCAGGTTTCTGA | 59.63 | 59.49 | 223 | 76 |
100.00%
|
100.00%
|
| Primer 1530 | Anaerocolumna | TACACCTGTCCGCTTGTGG | TGGCACGCAGGTTTCTGA | 59.63 | 59.49 | 225 | 76 |
100.00%
|
100.00%
|