| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 521 | Anaerocolumna | AAACAGCTGTCTCCGAGGC | GTTCCTTCCCCTGCTTTTGA | 60.00 | 58.00 | 265 | 99 |
100.00%
|
100.00%
|
| Primer 522 | Anaerocolumna | GCTGCTTCCGGTTTGATGG | ACCCATGTGCCAAGCTCA | 59.49 | 59.15 | 151 | 99 |
100.00%
|
100.00%
|
| Primer 523 | Anaerocolumna | ACAGGCTTGTGCTGCAGT | ACAATTGCGGAGAGGGTGT | 59.81 | 59.24 | 252 | 99 |
100.00%
|
100.00%
|
| Primer 524 | Anaerocolumna | ACAGGCTTGTGCTGCAGT | TTGCGGAGAGGGTGTCAT | 59.81 | 58.19 | 248 | 99 |
100.00%
|
100.00%
|
| Primer 525 | Anaerocolumna | TTGCACCCTCCAGCATGA | AACCGCAACAGCTGGACA | 58.83 | 59.81 | 193 | 99 |
100.00%
|
100.00%
|
| Primer 526 | Anaerocolumna | CGGTCACTTCCGGATGATGA | TGCCTGGCAGGAAAGGTA | 59.54 | 58.09 | 190 | 99 |
100.00%
|
100.00%
|
| Primer 527 | Anaerocolumna | TGTAGAAACCGCTCCAGCC | AACAGCAGAGGACGGATTCA | 59.70 | 59.02 | 214 | 99 |
100.00%
|
100.00%
|
| Primer 528 | Anaerocolumna | TGTAGAAACCGCTCCAGCC | CGATGAAACAGCAGAGGACG | 59.70 | 59.01 | 220 | 99 |
100.00%
|
100.00%
|
| Primer 529 | Anaerocolumna | ATGTGCCATGGCCAGATTGA | TTCAGCTCCTGCGCTTGT | 60.03 | 59.57 | 187 | 99 |
100.00%
|
100.00%
|
| Primer 530 | Anaerocolumna | AGGAAATGGTGGTGCTTCCA | TACAGGGGTTGCGTTGACG | 59.52 | 60.30 | 163 | 99 |
100.00%
|
100.00%
|