| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 31 | Anaerofustis | AGAAGCCAGTCCGTCTTGC | CCTTCGGCAAGAACGGAAC | 60.00 | 59.13 | 246 | 118 |
100.00%
|
100.00%
|
| Primer 32 | Anaerofustis | ACAGTGGAAGTCAATGACGGG | TCGGCACCTACTTCAAATCCA | 60.27 | 59.37 | 206 | 115 |
100.00%
|
50.00%
|
| Primer 33 | Anaerofustis | ATGACCAGGTAGCAGACGC | CCCTGTGCTGTATCGCCTT | 59.48 | 59.78 | 157 | 114 |
100.00%
|
100.00%
|
| Primer 34 | Anaerofustis | TCTTCGCTGGTAGTTGCCG | ATCTGCATTGGCTCCGCA | 60.08 | 59.73 | 201 | 113 |
100.00%
|
100.00%
|
| Primer 35 | Anaerofustis | GCTCACGCAGCTGATTTGC | TGCGCTGTCACCTAAATGC | 60.51 | 58.83 | 253 | 112 |
100.00%
|
50.00%
|
| Primer 36 | Anaerofustis | GCTCACGCAGCTGATTTGC | GTGCGCTGTCACCTAAATGC | 60.51 | 60.18 | 254 | 112 |
100.00%
|
50.00%
|
| Primer 37 | Anaerofustis | TCACGCAGCTGATTTGCAC | TGCGCTGTCACCTAAATGC | 59.42 | 58.83 | 251 | 112 |
100.00%
|
50.00%
|
| Primer 38 | Anaerofustis | TCACGCAGCTGATTTGCAC | GTGCGCTGTCACCTAAATGC | 59.42 | 60.18 | 252 | 112 |
100.00%
|
50.00%
|
| Primer 39 | Anaerofustis | ATGACCAGGTAGCAGACGC | TCCCTGTGCTGTATCGCCT | 59.48 | 60.69 | 158 | 111 |
100.00%
|
100.00%
|
| Primer 40 | Anaerofustis | ACGCGACAGCATTCAACG | ACAGGCACGATTTCAACTGC | 59.15 | 59.41 | 175 | 110 |
100.00%
|
100.00%
|