| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Anaerotignum | TGGGACACGCAACCATGT | ATCGCCAGGGTGAACAGTG | 59.49 | 60.00 | 158 | 148 |
100.00%
|
100.00%
|
| Primer 2 | Anaerotignum | TGGGACACGCAACCATGT | ACATCGCCAGGGTGAACAG | 59.49 | 60.00 | 160 | 148 |
100.00%
|
100.00%
|
| Primer 3 | Anaerotignum | TGGGACACGCAACCATGT | CATCGCCAGGGTGAACAGT | 59.49 | 60.00 | 159 | 143 |
100.00%
|
100.00%
|
| Primer 4 | Anaerotignum | TGGGACACGCAACCATGT | ATCGCCAGGGTGAACAGT | 59.49 | 58.52 | 158 | 143 |
100.00%
|
100.00%
|
| Primer 5 | Anaerotignum | TGGCAAAACCCAGGTGCA | ACCGTCAATGGTGGGCAT | 60.04 | 59.24 | 280 | 138 |
100.00%
|
100.00%
|
| Primer 6 | Anaerotignum | GCACCCAACGTTGACATGG | TCAGGTTTCTGCGCTGCA | 59.71 | 59.89 | 281 | 138 |
100.00%
|
100.00%
|
| Primer 7 | Anaerotignum | ACCCCAGTATGCACCCAAC | TCAGGTTTCTGCGCTGCA | 59.62 | 59.89 | 291 | 138 |
100.00%
|
100.00%
|
| Primer 8 | Anaerotignum | ACACAGGCTACATCGGTGG | TCAGGTTTCTGCGCTGCA | 59.40 | 59.89 | 184 | 138 |
100.00%
|
100.00%
|
| Primer 9 | Anaerotignum | GCCATTGCAGCCAACACA | TTGCTGCCAGTTCCGCAT | 59.26 | 60.28 | 199 | 137 |
100.00%
|
100.00%
|
| Primer 10 | Anaerotignum | TGGGACACGCAACCATGT | ACATCGCCAGGGTGAACA | 59.49 | 58.84 | 160 | 137 |
100.00%
|
100.00%
|