| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 51 | Anoxybacillus | TCCTTTCTCGCAGCAACCG | TTTTCAACGACCCCTCCCG | 60.67 | 59.93 | 180 | 115 |
100.00%
|
100.00%
|
| Primer 52 | Anoxybacillus | ACCATCCTTTCTCGCAGCA | TTTTCAACGACCCCTCCCG | 59.32 | 59.93 | 184 | 115 |
100.00%
|
100.00%
|
| Primer 53 | Anoxybacillus | TCGCTCGGGTTTGCTACA | TCGTAAAGCGAGCCACCA | 58.95 | 58.95 | 297 | 115 |
100.00%
|
100.00%
|
| Primer 54 | Anoxybacillus | ACGACCACTTGCTGCACTT | ACCCCTTCTTGCGCAACA | 60.15 | 59.81 | 210 | 115 |
100.00%
|
100.00%
|
| Primer 55 | Anoxybacillus | TGGCGACCGCATTCACTT | TCCATCGCTTTCCACGCT | 59.97 | 59.34 | 288 | 115 |
100.00%
|
100.00%
|
| Primer 56 | Anoxybacillus | TCCACAAGGCAATGCGGT | ACCTCCTCAATCTCGTAGCG | 59.88 | 58.97 | 195 | 114 |
100.00%
|
100.00%
|
| Primer 57 | Anoxybacillus | TCCACAAGGCAATGCGGT | ACGACAACCTCCTCAATCTCG | 59.88 | 59.80 | 201 | 114 |
100.00%
|
100.00%
|
| Primer 58 | Anoxybacillus | TCCACAAGGCAATGCGGT | AACCTCCTCAATCTCGTAGCG | 59.88 | 59.59 | 196 | 114 |
100.00%
|
100.00%
|
| Primer 59 | Anoxybacillus | ACGGGAAGCTTGTTTGCG | TTGGATGCCAAGCGAGCT | 58.97 | 59.65 | 200 | 114 |
100.00%
|
100.00%
|
| Primer 60 | Anoxybacillus | TGCGTCATCGGTGCAGAT | AGCGCTCCACGTTGTTGA | 59.42 | 59.89 | 281 | 112 |
100.00%
|
100.00%
|