| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 3541 | Azotobacter | TTCCGCATCGCCACCAAT | TTTCCAGCAGGCGTTCGA | 60.05 | 59.58 | 160 | 21 |
100.00%
|
100.00%
|
| Primer 3542 | Azotobacter | TTCCGCATCGCCACCAAT | ATTTCCAGCAGGCGTTCGA | 60.05 | 60.00 | 161 | 21 |
100.00%
|
100.00%
|
| Primer 3543 | Azotobacter | TGACGGCATCTCCATCGA | ATCGTCCGATCGACTCGTG | 58.07 | 59.36 | 211 | 21 |
100.00%
|
100.00%
|
| Primer 3544 | Azotobacter | ACTGCACCTTCTGGATGCC | GTTGGCGATGTTGTGCGA | 60.00 | 59.06 | 182 | 21 |
100.00%
|
100.00%
|
| Primer 3545 | Azotobacter | ATGTTCGGCTGCGAGCTT | CCATGATGGTGCCGATCGA | 60.05 | 59.93 | 175 | 21 |
100.00%
|
100.00%
|
| Primer 3546 | Azotobacter | ACCATCCGGTACTGGTCGA | GGCGTTCAGGATCAGGCTT | 60.00 | 60.08 | 287 | 21 |
100.00%
|
100.00%
|
| Primer 3547 | Azotobacter | ACCATCCGGTACTGGTCGA | TCAGGCTTCTCACGGCATG | 60.00 | 60.08 | 276 | 21 |
100.00%
|
100.00%
|
| Primer 3548 | Azotobacter | ACCATCCGGTACTGGTCGA | GATCAGGCTTCTCACGGCA | 60.00 | 59.78 | 278 | 21 |
100.00%
|
100.00%
|
| Primer 3549 | Azotobacter | TCGATACACGCAAGCGCA | GCATCGAAAGCACTGCGA | 60.13 | 58.83 | 160 | 21 |
100.00%
|
100.00%
|
| Primer 3550 | Azotobacter | GGACAGGCAACGCATAGGA | TGCGCTTGCGTGTATCGA | 59.78 | 60.13 | 215 | 21 |
100.00%
|
100.00%
|