| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 81 | Azotobacter | TGGAAGATGAACGGCAGCC | AGCCCAACTCCTCGAGCAT | 60.38 | 60.99 | 235 | 91 |
100.00%
|
100.00%
|
| Primer 82 | Azotobacter | ATGGCTGCCGAGCTGAAA | TCATCAGCACGTCGTCGAC | 59.65 | 60.15 | 259 | 90 |
100.00%
|
100.00%
|
| Primer 83 | Azotobacter | ATGGCTGCCGAGCTGAAA | AGAAGGACACGTCGAGCAC | 59.65 | 59.71 | 151 | 90 |
100.00%
|
100.00%
|
| Primer 84 | Azotobacter | CCCATGACGGCATCTCCAT | TCCGATCGACTCGTGGAGT | 59.55 | 60.08 | 211 | 90 |
100.00%
|
100.00%
|
| Primer 85 | Azotobacter | ACTGGAGATTCGCGAAGGC | TGGCTTCGACGATGCACA | 60.15 | 59.66 | 293 | 90 |
100.00%
|
100.00%
|
| Primer 86 | Azotobacter | TCGTCGTGCTGTTGCTGT | TGGAGATGCGCTGAAAGGC | 59.89 | 60.75 | 161 | 90 |
100.00%
|
100.00%
|
| Primer 87 | Azotobacter | TGTCAAGCGTGGCGTCAT | AAACTGACGCTTGCGCTG | 59.97 | 59.37 | 163 | 90 |
100.00%
|
100.00%
|
| Primer 88 | Azotobacter | TGCTGCATGGCGAGGAAA | AAGCGGACCTTGACGTAGC | 59.97 | 60.08 | 254 | 90 |
100.00%
|
100.00%
|
| Primer 89 | Azotobacter | CAGCCGTTCGCAGCAAAA | CAATCGGCAGGCGTTTCAT | 59.67 | 59.20 | 274 | 90 |
100.00%
|
100.00%
|
| Primer 90 | Azotobacter | CAGCCGTTCGCAGCAAAA | ATCGGCAGGCGTTTCATC | 59.67 | 58.19 | 272 | 90 |
100.00%
|
100.00%
|