| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1081 | Bacillus | TTGGTGACCCAACTCGCA | TGGGCAAACTTCACCTGGG | 59.09 | 60.15 | 212 | 65 |
100.00%
|
14.29%
|
| Primer 1082 | Bacillus | AGGCGACGATGAGCAACT | AGCGCACCTAAACCACCT | 59.03 | 58.84 | 294 | 65 |
100.00%
|
14.29%
|
| Primer 1083 | Bacillus | AGAGGAAGCGCAAGGGTT | CCGCATAAAAGCCTTAGCGC | 58.84 | 60.32 | 190 | 65 |
100.00%
|
15.48%
|
| Primer 1084 | Bacillus | CAGAGGAAGCGCAAGGGTT | CCGCATAAAAGCCTTAGCGC | 60.30 | 60.32 | 191 | 65 |
100.00%
|
16.67%
|
| Primer 1085 | Bacillus | TGGTTCGAGCAACTATGGGG | TCCTCTCCCACGAGACGTT | 59.75 | 59.93 | 258 | 65 |
100.00%
|
15.48%
|
| Primer 1086 | Bacillus | TGGGCAAAGCACACGGTT | ATAGCAACGCGTGGGTCA | 60.44 | 59.34 | 256 | 65 |
100.00%
|
13.10%
|
| Primer 1087 | Bacillus | TGGGCAAAGCACACGGTT | TAGCAACGCGTGGGTCAAT | 60.44 | 60.00 | 255 | 65 |
100.00%
|
15.48%
|
| Primer 1088 | Bacillus | TGGGCAAAGCACACGGTT | ATAGCAACGCGTGGGTCAA | 60.44 | 60.00 | 256 | 65 |
100.00%
|
15.48%
|
| Primer 1089 | Bacillus | TAGCTGCTGCACTCCGTT | TGAAGTGCGCTGTCATGGT | 58.94 | 59.93 | 205 | 65 |
100.00%
|
13.10%
|
| Primer 1090 | Bacillus | TGGCGATGGTTCAGAAGGA | TGCCTTCACCCAATTCCCT | 58.63 | 58.83 | 215 | 65 |
100.00%
|
16.67%
|