| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1221 | Bacillus | TTGGTGCTCATGCCGATGA | ACAAACCACGGTCTGGCA | 59.70 | 59.41 | 213 | 63 |
100.00%
|
16.67%
|
| Primer 1222 | Bacillus | TGGTATGTGACAGGACCGC | GCAGACTCTTTGCTTGTCCC | 59.41 | 59.12 | 185 | 63 |
100.00%
|
14.29%
|
| Primer 1223 | Bacillus | ATGTGACAGGACCGCACTT | GCAGACTCTTTGCTTGTCCC | 59.25 | 59.12 | 181 | 63 |
100.00%
|
14.29%
|
| Primer 1224 | Bacillus | AGGAAATGACGGGTGGACG | TGCCACTACCATCACGCTG | 59.70 | 60.08 | 210 | 63 |
100.00%
|
15.48%
|
| Primer 1225 | Bacillus | AGGAAATGACGGGTGGACG | GCCACTACCATCACGCTGA | 59.70 | 59.78 | 209 | 63 |
100.00%
|
15.48%
|
| Primer 1226 | Bacillus | ATTGCTGAAGCGCTTAGTGT | TCCACACCGATAATGCGCA | 58.19 | 59.78 | 188 | 63 |
100.00%
|
15.48%
|
| Primer 1227 | Bacillus | AGGTGTAATCCACGCAGCA | AGACGCATTGCTACCGCA | 59.32 | 59.74 | 156 | 63 |
100.00%
|
14.29%
|
| Primer 1228 | Bacillus | GGTGTAATCCACGCAGCAC | AGACGCATTGCTACCGCA | 59.21 | 59.74 | 155 | 63 |
100.00%
|
14.29%
|
| Primer 1229 | Bacillus | AGGTGTAATCGCAGCTGCA | GCCTCAACTAATGCAACCCC | 59.70 | 59.18 | 162 | 63 |
100.00%
|
15.48%
|
| Primer 1230 | Bacillus | TGCGATGGCTCTTGCACA | GGACATGGAACGATTTGCGC | 59.97 | 60.52 | 291 | 63 |
100.00%
|
13.10%
|