| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1311 | Bacillus | TGTGCTTGTATGCGATCGAGA | GGCAGCAGTATTGACCGCT | 59.87 | 60.45 | 289 | 61 |
100.00%
|
13.10%
|
| Primer 1312 | Bacillus | TGCCAAAGCGCTACAGTGA | TCCCCAAGCAAATACCCCA | 59.93 | 58.51 | 251 | 61 |
100.00%
|
15.48%
|
| Primer 1313 | Bacillus | TGCCAAAGCGCTACAGTG | TGTCCCCAAGCAAATACCCC | 58.35 | 59.96 | 253 | 61 |
100.00%
|
13.10%
|
| Primer 1314 | Bacillus | TGCCAAAGCGCTACAGTGA | GTCCCCAAGCAAATACCCC | 59.93 | 58.10 | 252 | 61 |
100.00%
|
13.10%
|
| Primer 1315 | Bacillus | ACAGTCTACCCGAAGCACG | TGTGTGCGATCCAGCGTA | 59.41 | 59.03 | 219 | 61 |
100.00%
|
14.29%
|
| Primer 1316 | Bacillus | TCGTGTACTTCGTTGCGGA | CCAATTCCAGCACCGTCAGA | 59.35 | 60.32 | 151 | 61 |
100.00%
|
15.48%
|
| Primer 1317 | Bacillus | TTGCACAAGAAGCAGCGG | CGAGTGCGGAATACTTTCGC | 58.97 | 59.70 | 175 | 61 |
100.00%
|
15.48%
|
| Primer 1318 | Bacillus | GCACAAGAAGCAGCGGAAG | CGAGTGCGGAATACTTTCGC | 59.79 | 59.70 | 173 | 61 |
100.00%
|
15.48%
|
| Primer 1319 | Bacillus | GCACAAGAAGCAGCGGAA | CGAGTGCGGAATACTTTCGC | 58.66 | 59.70 | 173 | 61 |
100.00%
|
15.48%
|
| Primer 1320 | Bacillus | GTTGCACAAGAAGCAGCGG | CGAGTGCGGAATACTTTCGC | 60.37 | 59.70 | 176 | 61 |
100.00%
|
15.48%
|