| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1921 | Bacillus | TGTTTCTTCCCGATTGCGC | TTCACTTGCACCGAGCCAT | 59.13 | 59.93 | 216 | 52 |
100.00%
|
15.48%
|
| Primer 1922 | Bacillus | ATGGCGATGGTTCAGAAGGA | TGCCTTCACCCAATTCCCTT | 59.09 | 59.51 | 216 | 52 |
100.00%
|
16.67%
|
| Primer 1923 | Bacillus | TGGCGATGGTTCAGAAGGA | ATGCCTTCACCCAATTCCCTT | 58.63 | 59.92 | 216 | 52 |
100.00%
|
16.67%
|
| Primer 1924 | Bacillus | TGTCGGTGCAGTTATCGCC | CTCCTGCTCCGTAACGCTT | 60.45 | 59.78 | 173 | 52 |
100.00%
|
13.10%
|
| Primer 1925 | Bacillus | TGCAGCAGGTGAAGGCTT | AACGCCACCTGTTGCTTG | 59.48 | 58.88 | 262 | 52 |
100.00%
|
13.10%
|
| Primer 1926 | Bacillus | TCCGATTCGCGTATCTCGATC | CAACCCACTGCTCGTCCTT | 59.87 | 59.93 | 266 | 52 |
100.00%
|
13.10%
|
| Primer 1927 | Bacillus | CCGATTCGCGTATCTCGATCA | CAACCCACTGCTCGTCCTT | 60.14 | 59.93 | 265 | 52 |
100.00%
|
13.10%
|
| Primer 1928 | Bacillus | ATGCATCGTCCAGCGCTT | CCAAGTGTAACACCGTTCCAA | 60.12 | 58.71 | 233 | 52 |
100.00%
|
15.48%
|
| Primer 1929 | Bacillus | TGCATCGTCCAGCGCTT | CCAAGTGTAACACCGTTCCA | 59.68 | 58.04 | 232 | 52 |
100.00%
|
15.48%
|
| Primer 1930 | Bacillus | TGCATCGTCCAGCGCTT | ACCAAGTGTAACACCGTTCC | 59.68 | 58.04 | 233 | 52 |
100.00%
|
15.48%
|