| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1951 | Bacillus | CGAGCTCGATGTCCTTGCT | GCGCTAAACGGCTTGAAGC | 59.86 | 60.51 | 272 | 51 |
100.00%
|
14.29%
|
| Primer 1952 | Bacillus | GCCGATTACGTTCCCGACA | CATCCCTACGCCAGCGAAT | 60.15 | 59.93 | 177 | 51 |
100.00%
|
15.48%
|
| Primer 1953 | Bacillus | AGCTGCTCGTCGTGCAAT | CGTAATGCTTCGCGTGCT | 60.05 | 58.92 | 213 | 51 |
100.00%
|
15.48%
|
| Primer 1954 | Bacillus | CTGTCGGCAGAACAAGAGTTT | ATCACTACCACCCTCCCCT | 58.79 | 58.88 | 228 | 51 |
100.00%
|
14.29%
|
| Primer 1955 | Bacillus | CTGTCGGCAGAACAAGAGTTT | TCATCACTACCACCCTCCCC | 58.79 | 60.33 | 230 | 51 |
100.00%
|
14.29%
|
| Primer 1956 | Bacillus | ACACCGCAACGTGAAGCA | AACGCTGCGCACCTTCTT | 60.51 | 60.59 | 223 | 51 |
100.00%
|
15.48%
|
| Primer 1957 | Bacillus | CCGCAACGTGAAGCAACA | AACGCTGCGCACCTTCTT | 59.29 | 60.59 | 220 | 51 |
100.00%
|
15.48%
|
| Primer 1958 | Bacillus | CACCGCAACGTGAAGCAA | AACGCTGCGCACCTTCTT | 59.29 | 60.59 | 222 | 51 |
100.00%
|
15.48%
|
| Primer 1959 | Bacillus | ACCGCAACGTGAAGCAAC | AACGCTGCGCACCTTCTT | 59.29 | 60.59 | 221 | 51 |
100.00%
|
15.48%
|
| Primer 1960 | Bacillus | AACACCGCAACGTGAAGC | AACGCTGCGCACCTTCTT | 59.29 | 60.59 | 224 | 51 |
100.00%
|
15.48%
|